Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 423885..424467 Replicon chromosome
Accession NC_018221
Organism Enterococcus faecalis D32

Toxin (Protein)


Gene name zeta Uniprot ID A0A0M2AE74
Locus tag EFD32_RS02285 Protein ID WP_002355414.1
Coordinates 424159..424467 (+) Length 103 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID Q9AL19
Locus tag EFD32_RS02280 Protein ID WP_002326825.1
Coordinates 423885..424157 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EFD32_RS02255 419721..420475 + 755 Protein_406 IS21-like element helper ATPase IstB -
EFD32_RS02270 422728..423582 + 855 WP_002364921.1 ParA family protein -
EFD32_RS02275 423653..423868 + 216 WP_002355411.1 peptide-binding protein -
EFD32_RS02280 423885..424157 + 273 WP_002326825.1 antitoxin Antitoxin
EFD32_RS02285 424159..424467 + 309 WP_002355414.1 zeta toxin family protein Toxin
EFD32_RS15655 424547..424975 - 429 WP_071623404.1 tyrosine-type recombinase/integrase -
EFD32_RS02290 425020..425520 - 501 WP_002355415.1 HAD family hydrolase -
EFD32_RS02295 425525..426292 - 768 WP_002355416.1 DeoR/GlpR family DNA-binding transcription regulator -
EFD32_RS02300 426780..427205 + 426 WP_002355418.1 galactose-6-phosphate isomerase subunit LacA -
EFD32_RS02305 427222..427737 + 516 WP_002345825.1 galactose-6-phosphate isomerase subunit LacB -
EFD32_RS02310 427748..428680 + 933 WP_002363040.1 tagatose-6-phosphate kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-99)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11525.91 Da        Isoelectric Point: 5.7251

>T28124 WP_002355414.1 NC_018221:424159-424467 [Enterococcus faecalis D32]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELV
KLYEKDVVKHATSYSNQLVKLN

Download         Length: 309 bp

>T28124 NC_018221:424159-424467 [Enterococcus faecalis D32]
ATGGCGAATATTACTGACTTCACCGAAAAGCAATTTGAAGATCGTTTAGAAAAGAATGTTGAACGACTAACTAAAAATAG
ACTAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGACAACCAGGATCAGGGAAAACTAGTTTGCGATCGGCAATTT
CCGAAGAAACACAAGGAAATGTTGTTATCATTGATAATGATACGTTCAAACAACAGCACCCAAATTTTGACGAATTAGTG
AAACTCTATGAAAAAGACGTAGTAAAACATGCAACATCCTATTCTAATCAGCTGGTAAAGCTTAATTAG

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10720.17 Da        Isoelectric Point: 4.4865

>AT28124 WP_002326825.1 NC_018221:423885-424157 [Enterococcus faecalis D32]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILTKEKVA

Download         Length: 273 bp

>AT28124 NC_018221:423885-424157 [Enterococcus faecalis D32]
ATGGCAGTTACGTATGAAAAAACATTTGAAATTGAAATCATTAATGAGTTATCGGCAAGTGTTTATAATCGGGTATTAAA
TTATGTTTTGAATCATGAACTAGATACTAAAAATACTCGTTTACTAGAAGTGAATCTTTTAAATCAATTAGAAGTGGCAC
AAGAAGTTGATTTATTTCAACAACCATTTGAAGAATTACAAGCTATTCATGAGTATTGGCGGTCAATGAATCAATATTCA
AAACAAATTTTGACAAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2AE74

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AF93

References