Detailed information of TA system TA028121

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 5706548..5707431
• Replicon: chromosome
• Accession: NC_018220
• Organism: Pseudomonas putida DOT-T1E

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A2K4LSC9
• Locus tag: T1E_RS26850
• Protein ID: WP_012052908.1
• Coordinates: 5706548..5706985 (-)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: I7C3Q7
• Locus tag: T1E_RS26855
• Protein ID: WP_003250525.1
• Coordinates: 5706982..5707431 (-)
• Length: 150 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
T1E_RS26830	5701929..5702685	-	757	Protein_5239	PAAR domain-containing protein	-
T1E_RS26835	5702849..5704078	-	1230	WP_014861774.1	acyl-CoA dehydrogenase	-
T1E_RS26840	5704219..5705142	+	924	WP_014753760.1	LysR family transcriptional regulator	-
T1E_RS26845	5705336..5706490	+	1155	WP_060537721.1	aminotransferase class V-fold PLP-dependent enzyme	-
T1E_RS26850	5706548..5706985	-	438	WP_012052908.1	RES family NAD+ phosphorylase	Toxin
T1E_RS26855	5706982..5707431	-	450	WP_003250525.1	DUF2384 domain-containing protein	Antitoxin
T1E_RS26860	5707574..5708227	-	654	WP_004573726.1	oxygen-insensitive NAD(P)H-dependent nitroreductase NfsB	-
T1E_RS26865	5708331..5709254	+	924	WP_014861777.1	LysR family transcriptional regulator	-
T1E_RS26870	5709303..5710133	+	831	WP_003250518.1	AraC family transcriptional regulator	-
T1E_RS26875	5710185..5710769	+	585	WP_004573728.1	LysE family transporter	-
T1E_RS26880	5710809..5712008	-	1200	WP_014861778.1	sugar transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-138)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (33-91)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15944.21 Da
• Isoelectric Point: 5.6016

>T28121 WP_012052908.1 NC_018220:c5706985-5706548 [Pseudomonas putida DOT-T1E]
VILWRISAYADLSGTGGLRVSGRWHQAGRPVVYAATSPPGAMLEVLVHLEIDPEDFPTTMRLLRIELPDTVSQAQLPALQ
PGWSAQPELTRTLGNRFLDDGSALLLPVPSAIMPSTTNYLFNPRHPQAQSAKIQVEDFTPDSRLF

Nucleotide

• Length: 438 bp

>T28121 NC_018220:c5706985-5706548 [Pseudomonas putida DOT-T1E]
GTGATTTTGTGGCGAATCAGCGCCTATGCAGACTTGAGTGGTACGGGGGGCTTGCGTGTAAGTGGCCGCTGGCACCAGGC
GGGTCGGCCGGTGGTGTATGCCGCCACCAGCCCTCCCGGGGCGATGCTGGAAGTGTTGGTGCACCTGGAGATCGACCCCG
AGGATTTCCCGACCACCATGCGCTTGCTTCGCATCGAACTGCCCGACACGGTTTCCCAGGCGCAGTTGCCCGCTTTGCAG
CCTGGCTGGTCTGCTCAGCCTGAGTTGACCCGTACGCTGGGCAATCGCTTTCTGGATGACGGTTCGGCATTGCTGCTGCC
GGTGCCAAGCGCGATCATGCCCAGCACCACCAATTACCTGTTCAACCCACGGCACCCGCAGGCGCAGAGCGCGAAAATCC
AGGTTGAGGATTTCACCCCGGACAGCCGCCTGTTCTAG

Antitoxin

Protein

• Length: 150 a.a.
• Molecular weight: 16956.46 Da
• Isoelectric Point: 5.7258

>AT28121 WP_003250525.1 NC_018220:c5707431-5706982 [Pseudomonas putida DOT-T1E]
MLAEVLRDNGYHEYRARLQALLDIPELASDFEIHTRITDGFAATWLVKLTERGVLTPVERDQIIPLRTLKSRIERDQPLT
VDESDRLFRSAHITAMAEAVFGEAGKAKRWLSKPKERFSGLTPMQMLTTQQGTTQVEEMLLQIAEGYGL

Nucleotide

• Length: 450 bp

>AT28121 NC_018220:c5707431-5706982 [Pseudomonas putida DOT-T1E]
ATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCGGGCGCGCTTGCAGGCGCTGCTGGACATTCCCGAGCT
TGCCAGTGATTTCGAAATCCACACCCGCATCACCGATGGTTTTGCCGCCACCTGGTTGGTAAAGCTGACCGAACGCGGTG
TGCTCACGCCGGTGGAGCGCGACCAGATCATCCCGCTGCGCACCCTCAAATCCCGTATCGAGCGCGACCAACCGCTGACC
GTGGATGAGAGCGATCGGCTGTTCCGCTCGGCCCACATCACCGCCATGGCCGAGGCTGTGTTCGGTGAAGCGGGCAAGGC
CAAACGCTGGCTTTCCAAGCCCAAGGAGCGTTTCTCGGGGCTGACGCCGATGCAGATGCTCACCACCCAGCAAGGCACCA
CGCAGGTCGAAGAAATGCTGCTGCAGATTGCCGAGGGCTATGGCCTGTGA

Structures

Toxin

• AlphaFold DB: A0A2K4LSC9

Antitoxin

• AlphaFold DB: I7C3Q7