28120

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-PumB/upstrm_HI1419-dnstrm_HI1420
Location 5046079..5046665 Replicon chromosome
Accession NC_018220
Organism Pseudomonas putida DOT-T1E

Toxin (Protein)


Gene name relE Uniprot ID -
Locus tag T1E_RS23535 Protein ID WP_128573832.1
Coordinates 5046079..5046324 (+) Length 82 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID -
Locus tag T1E_RS23540 Protein ID WP_014861463.1
Coordinates 5046369..5046665 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
T1E_RS23510 5041680..5042534 + 855 WP_014861459.1 putative selenate ABC transporter substrate-binding protein -
T1E_RS23515 5042531..5043340 + 810 WP_014861460.1 phosphonate ABC transporter ATP-binding protein -
T1E_RS23520 5043334..5044161 + 828 WP_004576350.1 ABC transporter permease subunit -
T1E_RS23525 5044158..5044925 + 768 WP_014861461.1 phosphonate ABC transporter, permease protein PhnE -
T1E_RS23530 5044962..5045855 - 894 WP_014861462.1 hypothetical protein -
T1E_RS23535 5046079..5046324 + 246 WP_128573832.1 addiction module antitoxin RelB Toxin
T1E_RS23540 5046369..5046665 + 297 WP_014861463.1 putative addiction module antidote protein Antitoxin
T1E_RS23545 5046676..5047155 - 480 WP_014861464.1 GNAT family N-acetyltransferase -
T1E_RS23550 5047264..5047911 - 648 WP_121782881.1 serine protease -
T1E_RS23555 5048255..5048677 + 423 WP_012051239.1 VOC family protein -
T1E_RS23560 5049263..5050654 + 1392 WP_014861466.1 FAD-binding oxidoreductase -
T1E_RS23565 5050734..5051231 + 498 WP_014589747.1 RidA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(2-81)

Antitoxin

Uncharacterized protein HI1420

(5-91)


Sequences

Toxin        


Download         Length: 82 a.a.        Molecular weight: 8927.34 Da        Isoelectric Point: 10.6759

>T28120 WP_128573832.1 NC_018220:5046079-5046324 [Pseudomonas putida DOT-T1E]
MFLVEQTESFAAWLSSLRDLRARLAIGRRLERAAAGNLGDFKWLGGGIGELRIDVAAGYRVYFTQKGQRLVLLLVGGDKS
T

Download         Length: 246 bp

>T28120 NC_018220:5046079-5046324 [Pseudomonas putida DOT-T1E]
ATGTTTCTCGTTGAACAGACGGAAAGCTTTGCCGCTTGGCTATCGAGCTTGAGAGATTTGAGGGCCAGGCTGGCGATAGG
TCGTAGGCTGGAGCGAGCAGCGGCGGGCAATCTTGGTGACTTCAAGTGGCTTGGAGGCGGTATTGGTGAGCTTCGCATCG
ACGTAGCTGCCGGTTATCGCGTCTATTTCACGCAAAAGGGGCAGAGGTTGGTTTTACTGCTGGTCGGCGGCGACAAGTCC
ACCTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 10706.42 Da        Isoelectric Point: 6.7249

>AT28120 WP_014861463.1 NC_018220:5046369-5046665 [Pseudomonas putida DOT-T1E]
MSEKLIPFDMAALLSSDEAISEYLSQVLSNGDTEEIIRALGHIARARGMTHIAMESGLGRESLYKALSPGAKPRFDTVLR
VIRALGVDLLVQPHAMTR

Download         Length: 297 bp

>AT28120 NC_018220:5046369-5046665 [Pseudomonas putida DOT-T1E]
ATGAGCGAAAAATTGATCCCGTTTGATATGGCAGCCTTGTTGAGCAGCGATGAGGCAATCAGTGAATACCTTAGTCAGGT
GCTATCAAATGGCGATACCGAGGAAATCATTCGAGCACTTGGCCATATCGCTCGTGCACGGGGCATGACACATATTGCTA
TGGAGAGTGGGCTGGGTAGAGAAAGCCTGTACAAGGCGCTGAGCCCTGGCGCGAAGCCTCGTTTTGACACTGTATTGAGG
GTCATTCGTGCTCTGGGGGTGGACCTTCTCGTTCAGCCACATGCAATGACCCGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References