Detailed information of TA system TA281180

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 120184..120709
• Replicon: plasmid unnamed3
• Accession: NZ_CP124743
• Organism: Escherichia coli O157:H7 str. EDL933

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: V0SSI5
• Locus tag: QI555_RS28995
• Protein ID: WP_001159868.1
• Coordinates: 120404..120709 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1PPD8
• Locus tag: QI555_RS28990
• Protein ID: WP_000813634.1
• Coordinates: 120184..120402 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QI555_RS28960 (115467)	115467..115697	+	231	WP_010891288.1	hypothetical protein	-
QI555_RS28965 (115818)	115818..116558	+	741	WP_001066920.1	tyrosine-type recombinase/integrase	-
QI555_RS28970 (116843)	116843..117820	-	978	WP_000361615.1	RepB family plasmid replication initiator protein	-
QI555_RS28975 (118228)	118228..118428	-	201	WP_000708307.1	hypothetical protein	-
QI555_RS28980 (118425)	118425..119045	-	621	WP_001248529.1	hypothetical protein	-
QI555_RS28985 (119042)	119042..119725	-	684	WP_010891291.1	DNA-binding protein	-
QI555_RS28990 (120184)	120184..120402	+	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
QI555_RS28995 (120404)	120404..120709	+	306	WP_001159868.1	type II toxin-antitoxin system toxin CcdB	Toxin
QI555_RS29000 (120710)	120710..121516	+	807	WP_000016989.1	site-specific integrase	-
QI555_RS29010 (122778)	122778..123116	+	339	WP_071525396.1	RepB family plasmid replication initiator protein	-
QI555_RS29015 (123704)	123704..124870	+	1167	WP_000772446.1	plasmid-partitioning protein SopA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	toxB / espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD	1..136590	136590

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11706.51 Da
• Isoelectric Point: 6.4674

>T281180 WP_001159868.1 NZ_CP124743:120404-120709 [Escherichia coli O157:H7 str. EDL933]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T281180 NZ_CP124743:120404-120709 [Escherichia coli O157:H7 str. EDL933]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.34 Da
• Isoelectric Point: 4.7405

>AT281180 WP_000813634.1 NZ_CP124743:120184-120402 [Escherichia coli O157:H7 str. EDL933]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT281180 NZ_CP124743:120184-120402 [Escherichia coli O157:H7 str. EDL933]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Structures

Toxin

• PDB: 4VUB
• PDB: 2VUB
• PDB: 7EPI
• PDB: 7EPG
• PDB: 3VUB
• PDB: 1X75
• PDB: 1VUB
• PDB: 7EPJ
• PDB: 3HPW
• PDB: 3G7Z

Antitoxin

• PDB: 3TCJ
• PDB: 2H3A
• PDB: 2ADN
• PDB: 2ADL
• PDB: 3HPW
• PDB: 2H3C
• PDB: 3G7Z
• AlphaFold DB: A0A829CQY2