Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3601502..3602156 Replicon chromosome
Accession NZ_CP124740
Organism Escherichia coli O157:H7 str. EDL933

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag QI555_RS17790 Protein ID WP_000244781.1
Coordinates 3601749..3602156 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag QI555_RS17785 Protein ID WP_000354046.1
Coordinates 3601502..3601768 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QI555_RS17765 (3597590) 3597590..3599023 - 1434 WP_001310226.1 6-phospho-beta-glucosidase BglA -
QI555_RS17770 (3599068) 3599068..3599379 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
QI555_RS17775 (3599543) 3599543..3600202 + 660 WP_000250274.1 hemolysin III family protein -
QI555_RS17780 (3600279) 3600279..3601259 - 981 WP_000886053.1 tRNA-modifying protein YgfZ -
QI555_RS17785 (3601502) 3601502..3601768 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
QI555_RS17790 (3601749) 3601749..3602156 + 408 WP_000244781.1 protein YgfX Toxin
QI555_RS17795 (3602196) 3602196..3602717 - 522 WP_001055874.1 flavodoxin FldB -
QI555_RS17800 (3602829) 3602829..3603725 + 897 WP_000806640.1 site-specific tyrosine recombinase XerD -
QI555_RS17805 (3603750) 3603750..3604460 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
QI555_RS17810 (3604466) 3604466..3606199 + 1734 WP_000813185.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T281164 WP_000244781.1 NZ_CP124740:3601749-3602156 [Escherichia coli O157:H7 str. EDL933]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT281164 WP_000354046.1 NZ_CP124740:3601502-3601768 [Escherichia coli O157:H7 str. EDL933]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References