Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 3063071..3063871 Replicon chromosome
Accession NZ_CP124740
Organism Escherichia coli O157:H7 str. EDL933

Toxin (Protein)


Gene name ataT Uniprot ID Q8XED1
Locus tag QI555_RS15040 Protein ID WP_000342450.1
Coordinates 3063344..3063871 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag QI555_RS15035 Protein ID WP_001277108.1
Coordinates 3063071..3063337 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QI555_RS15015 (3058729) 3058729..3059397 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
QI555_RS15020 (3059390) 3059390..3060448 + 1059 WP_001042018.1 permease-like cell division protein FtsX -
QI555_RS15025 (3060693) 3060693..3061547 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
QI555_RS15030 (3061818) 3061818..3062921 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
QI555_RS15035 (3063071) 3063071..3063337 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
QI555_RS15040 (3063344) 3063344..3063871 + 528 WP_000342450.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
QI555_RS15045 (3063868) 3063868..3064251 - 384 WP_000778769.1 aspartate 1-decarboxylase autocleavage activator PanM -
QI555_RS15050 (3064675) 3064675..3065784 + 1110 WP_001301528.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
QI555_RS15055 (3065832) 3065832..3066758 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
QI555_RS15060 (3066755) 3066755..3068032 + 1278 WP_000803797.1 branched chain amino acid ABC transporter permease LivM -
QI555_RS15065 (3068029) 3068029..3068796 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19678.61 Da        Isoelectric Point: 6.9489

>T281160 WP_000342450.1 NZ_CP124740:3063344-3063871 [Escherichia coli O157:H7 str. EDL933]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMNVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT281160 WP_001277108.1 NZ_CP124740:3063071-3063337 [Escherichia coli O157:H7 str. EDL933]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7CHD

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References