Detailed information of TA system TA028109

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 3136630..3137234
• Replicon: chromosome
• Accession: NC_018143
• Organism: Mycobacterium tuberculosis H37Rv

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P71623
• Locus tag: RVBD_RS14940
• Protein ID: WP_003414492.1
• Coordinates: 3136630..3137022 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P71622
• Locus tag: RVBD_RS14945
• Protein ID: WP_003912003.1
• Coordinates: 3137019..3137234 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RVBD_RS14910	3131780..3132568	-	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-
RVBD_RS14915	3132902..3133447	-	546	WP_003913758.1	DUF1802 family protein	-
RVBD_RS14920	3133719..3134603	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
RVBD_RS14925	3134606..3135493	-	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
RVBD_RS14930	3135798..3136343	-	546	WP_003899500.1	DUF1802 family protein	-
RVBD_RS14935	3136340..3136609	-	270	WP_003414489.1	DUF2277 family protein	-
RVBD_RS14940	3136630..3137022	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	Toxin
RVBD_RS14945	3137019..3137234	-	216	WP_003912003.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
RVBD_RS14950	3137281..3138030	+	750	WP_003414497.1	enoyl-CoA hydratase	-
RVBD_RS14955	3138109..3139191	-	1083	WP_003414499.1	ABC transporter ATP-binding protein	-
RVBD_RS14960	3139184..3140494	-	1311	WP_003414501.1	ABC transporter substrate-binding protein	-
RVBD_RS14965	3140497..3141324	-	828	WP_003414504.1	carbohydrate ABC transporter permease	-
RVBD_RS14970	3141321..3142232	-	912	WP_003414505.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14610.74 Da
• Isoelectric Point: 6.7594

>T28109 WP_003414492.1 NC_018143:c3137022-3136630 [Mycobacterium tuberculosis H37Rv]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T28109 NC_018143:c3137022-3136630 [Mycobacterium tuberculosis H37Rv]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7483.50 Da
• Isoelectric Point: 4.9732

>AT28109 WP_003912003.1 NC_018143:c3137234-3137019 [Mycobacterium tuberculosis H37Rv]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAADDDELFTTGVSWNVS

Nucleotide

• Length: 216 bp

>AT28109 NC_018143:c3137234-3137019 [Mycobacterium tuberculosis H37Rv]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGCGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Structures

Toxin

• AlphaFold DB: A0A7U4BWM8

Antitoxin

• AlphaFold DB: P71622