28107

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3110177..3110747 Replicon chromosome
Accession NC_018143
Organism Mycobacterium tuberculosis H37Rv

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag RVBD_RS14790 Protein ID WP_003414166.1
Coordinates 3110177..3110533 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag RVBD_RS14795 Protein ID WP_003901465.1
Coordinates 3110517..3110747 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RVBD_RS14770 3105629..3107317 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
RVBD_RS14775 3107321..3107647 - 327 WP_003414157.1 hypothetical protein -
RVBD_RS14780 3107820..3108407 + 588 WP_003914429.1 DUF3558 family protein -
RVBD_RS14785 3108426..3110075 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
RVBD_RS14790 3110177..3110533 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
RVBD_RS14795 3110517..3110747 - 231 WP_003901465.1 antitoxin MazE Antitoxin
RVBD_RS14800 3110790..3111833 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
RVBD_RS22225 3112024..3112299 + 276 WP_003911993.1 DUF1778 domain-containing protein -
RVBD_RS22525 3112475..3112729 - 255 WP_003917684.1 hypothetical protein -
RVBD_RS14815 3112877..3113281 + 405 WP_009938577.1 hypothetical protein -
RVBD_RS14820 3113278..3113469 + 192 WP_003414184.1 hypothetical protein -
RVBD_RS14825 3113533..3114822 + 1290 Protein_2909 transposase family protein -
RVBD_RS14830 3115056..3115313 + 258 WP_003899489.1 hypothetical protein -
RVBD_RS14835 3115418..3115729 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T28107 WP_003414166.1 NC_018143:c3110533-3110177 [Mycobacterium tuberculosis H37Rv]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T28107 NC_018143:c3110533-3110177 [Mycobacterium tuberculosis H37Rv]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT28107 WP_003901465.1 NC_018143:c3110747-3110517 [Mycobacterium tuberculosis H37Rv]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT28107 NC_018143:c3110747-3110517 [Mycobacterium tuberculosis H37Rv]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References