28102

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2869737..2870374 Replicon chromosome
Accession NC_018143
Organism Mycobacterium tuberculosis H37Rv

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag RVBD_RS13495 Protein ID WP_003413180.1
Coordinates 2869737..2870132 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag RVBD_RS13500 Protein ID WP_003413183.1
Coordinates 2870129..2870374 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RVBD_RS13455 2865140..2866351 + 1212 WP_009936235.1 alpha/beta hydrolase family protein -
RVBD_RS13460 2866478..2867137 + 660 WP_003900846.1 LppA family lipoprotein -
RVBD_RS13465 2867134..2867796 + 663 WP_003900848.1 hypothetical protein -
RVBD_RS13470 2867793..2868071 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
RVBD_RS13475 2868164..2868577 + 414 WP_003413164.1 PIN domain nuclease -
RVBD_RS13480 2868616..2868873 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
RVBD_RS13485 2868870..2869247 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
RVBD_RS13490 2869263..2869637 - 375 WP_003413177.1 hypothetical protein -
RVBD_RS13495 2869737..2870132 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
RVBD_RS13500 2870129..2870374 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
RVBD_RS13505 2870785..2871204 - 420 WP_003413190.1 A24 family peptidase -
RVBD_RS13510 2871216..2872025 - 810 WP_003413193.1 shikimate dehydrogenase -
RVBD_RS13515 2872022..2873275 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
RVBD_RS13520 2873268..2873780 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T28102 WP_003413180.1 NC_018143:c2870132-2869737 [Mycobacterium tuberculosis H37Rv]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T28102 NC_018143:c2870132-2869737 [Mycobacterium tuberculosis H37Rv]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT28102 WP_003413183.1 NC_018143:c2870374-2870129 [Mycobacterium tuberculosis H37Rv]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT28102 NC_018143:c2870374-2870129 [Mycobacterium tuberculosis H37Rv]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References