Detailed information of TA system TA028101

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2868616..2869247
• Replicon: chromosome
• Accession: NC_018143
• Organism: Mycobacterium tuberculosis H37Rv

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: RVBD_RS13485
• Protein ID: WP_003413174.1
• Coordinates: 2868870..2869247 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: RVBD_RS13480
• Protein ID: WP_003413167.1
• Coordinates: 2868616..2868873 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RVBD_RS13450	2864530..2864844	+	315	WP_009937839.1	hypothetical protein	-
RVBD_RS13455	2865140..2866351	+	1212	WP_009936235.1	alpha/beta hydrolase family protein	-
RVBD_RS13460	2866478..2867137	+	660	WP_003900846.1	LppA family lipoprotein	-
RVBD_RS13465	2867134..2867796	+	663	WP_003900848.1	hypothetical protein	-
RVBD_RS13470	2867793..2868071	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
RVBD_RS13475	2868164..2868577	+	414	WP_003413164.1	PIN domain nuclease	-
RVBD_RS13480	2868616..2868873	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	Antitoxin
RVBD_RS13485	2868870..2869247	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
RVBD_RS13490	2869263..2869637	-	375	WP_003413177.1	hypothetical protein	-
RVBD_RS13495	2869737..2870132	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
RVBD_RS13500	2870129..2870374	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
RVBD_RS13505	2870785..2871204	-	420	WP_003413190.1	A24 family peptidase	-
RVBD_RS13510	2871216..2872025	-	810	WP_003413193.1	shikimate dehydrogenase	-
RVBD_RS13515	2872022..2873275	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
RVBD_RS13520	2873268..2873780	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T28101 WP_003413174.1 NC_018143:2868870-2869247 [Mycobacterium tuberculosis H37Rv]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T28101 NC_018143:2868870-2869247 [Mycobacterium tuberculosis H37Rv]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT28101 WP_003413167.1 NC_018143:2868616-2868873 [Mycobacterium tuberculosis H37Rv]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT28101 NC_018143:2868616-2868873 [Mycobacterium tuberculosis H37Rv]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A829C9W5