Detailed information of TA system TA280980

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 6834392..6834897
• Replicon: chromosome
• Accession: NZ_CP124655
• Organism: Pseudomonas aeruginosa strain 2022CK-00096

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: P7I95_RS32450
• Protein ID: WP_003083773.1
• Coordinates: 6834616..6834897 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: P7I95_RS32445
• Protein ID: WP_003083775.1
• Coordinates: 6834392..6834619 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P7I95_RS32420 (P7I95_32420)	6829408..6830901	+	1494	WP_003101230.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-
P7I95_RS32425 (P7I95_32425)	6831070..6832497	+	1428	WP_003083784.1	GABA permease	-
P7I95_RS32430 (P7I95_32430)	6832581..6832922	-	342	WP_014603467.1	zinc ribbon domain-containing protein YjdM	-
P7I95_RS32435 (P7I95_32435)	6832995..6833495	-	501	WP_003083778.1	LEA type 2 family protein	-
P7I95_RS32440 (P7I95_32440)	6833596..6834216	+	621	WP_003101226.1	hypothetical protein	-
P7I95_RS32445 (P7I95_32445)	6834392..6834619	+	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
P7I95_RS32450 (P7I95_32450)	6834616..6834897	+	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
P7I95_RS32455 (P7I95_32455)	6835197..6836105	+	909	WP_003083769.1	LysR family transcriptional regulator	-
P7I95_RS32460 (P7I95_32460)	6836137..6836547	-	411	WP_003110659.1	aegerolysin family protein	-
P7I95_RS32465 (P7I95_32465)	6836727..6837461	-	735	WP_003083764.1	GntR family transcriptional regulator	-
P7I95_RS32470 (P7I95_32470)	6837562..6838248	-	687	WP_003083762.1	FadR/GntR family transcriptional regulator	-
P7I95_RS32475 (P7I95_32475)	6838297..6839646	-	1350	WP_003137006.1	C4-dicarboxylate transporter DctA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T280980 WP_003083773.1 NZ_CP124655:6834616-6834897 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T280980 NZ_CP124655:6834616-6834897 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT280980 WP_003083775.1 NZ_CP124655:6834392-6834619 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT280980 NZ_CP124655:6834392-6834619 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9