Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tad-ata/COG5606-COG4679
Location 6710742..6711435 Replicon chromosome
Accession NZ_CP124646
Organism Pseudomonas aeruginosa strain 2020CK-00185

Toxin (Protein)


Gene name tad Uniprot ID N2IHR9
Locus tag P7I78_RS31265 Protein ID WP_003151133.1
Coordinates 6710742..6711119 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name ata Uniprot ID N2IIN5
Locus tag P7I78_RS31270 Protein ID WP_001172026.1
Coordinates 6711100..6711435 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P7I78_RS31245 (P7I78_31245) 6705895..6706440 - 546 WP_182330315.1 hypothetical protein -
P7I78_RS31250 (P7I78_31250) 6706433..6706723 - 291 WP_225850518.1 hypothetical protein -
P7I78_RS31255 (P7I78_31255) 6706935..6709964 - 3030 WP_065761565.1 Tn3 family transposase -
P7I78_RS31260 (P7I78_31260) 6709948..6710550 - 603 WP_010465829.1 recombinase family protein -
P7I78_RS31265 (P7I78_31265) 6710742..6711119 + 378 WP_003151133.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
P7I78_RS31270 (P7I78_31270) 6711100..6711435 + 336 WP_001172026.1 helix-turn-helix transcriptional regulator Antitoxin
P7I78_RS31275 (P7I78_31275) 6711450..6711785 + 336 WP_000741275.1 hypothetical protein -
P7I78_RS31280 (P7I78_31280) 6711809..6712135 + 327 WP_000091614.1 hypothetical protein -
P7I78_RS31285 (P7I78_31285) 6712132..6712512 + 381 WP_001054412.1 hypothetical protein -
P7I78_RS31290 (P7I78_31290) 6712669..6713052 + 384 WP_282415613.1 DUF3391 domain-containing protein -
P7I78_RS31295 (P7I78_31295) 6713136..6714005 + 870 WP_223198360.1 HD-GYP domain-containing protein -
P7I78_RS31300 (P7I78_31300) 6714779..6715186 - 408 WP_023383747.1 hypothetical protein -
P7I78_RS31305 (P7I78_31305) 6715223..6715915 - 693 WP_023383746.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element blaIMP-15 / blaOXA-2 / catB2 - 6640653..6900395 259742


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-109)

Antitoxin

Cro/C1-type helix-turn-helix domain

(40-93)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13705.78 Da        Isoelectric Point: 9.4693

>T280936 WP_003151133.1 NZ_CP124646:6710742-6711119 [Pseudomonas aeruginosa]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKVLKGFGGAGVLEVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQELRNAKTNH

Download         Length: 378 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12201.39 Da        Isoelectric Point: 10.6058

>AT280936 WP_001172026.1 NZ_CP124646:6711100-6711435 [Pseudomonas aeruginosa]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A024ELN5

Antitoxin

Source ID Structure
AlphaFold DB A0A024EKI7

References