Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2234313..2234899 Replicon chromosome
Accession NC_018143
Organism Mycobacterium tuberculosis H37Rv

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag RVBD_RS10470 Protein ID WP_003410010.1
Coordinates 2234313..2234657 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag RVBD_RS10475 Protein ID WP_003410014.1
Coordinates 2234651..2234899 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RVBD_RS10435 2230019..2230618 + 600 WP_003409989.1 L-lysine exporter -
RVBD_RS10440 2231034..2231462 + 429 WP_003409992.1 cellulose-binding protein -
RVBD_RS10445 2231688..2232227 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
RVBD_RS10450 2232747..2233307 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
RVBD_RS10455 2233304..2233645 - 342 WP_003410003.1 DUF2384 domain-containing protein -
RVBD_RS10460 2233731..2233988 + 258 WP_003410006.1 hypothetical protein -
RVBD_RS10465 2233889..2234224 - 336 WP_003410009.1 dehydrogenase -
RVBD_RS10470 2234313..2234657 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
RVBD_RS10475 2234651..2234899 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
RVBD_RS10480 2234999..2237314 - 2316 WP_003899120.1 cation transporter ATPase CptG -
RVBD_RS10485 2237311..2237583 - 273 WP_003410017.1 DUF1490 family protein -
RVBD_RS10490 2237636..2237992 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
RVBD_RS10495 2238149..2238916 + 768 WP_003410019.1 hemerythrin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T28092 WP_003410010.1 NC_018143:c2234657-2234313 [Mycobacterium tuberculosis H37Rv]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T28092 NC_018143:c2234657-2234313 [Mycobacterium tuberculosis H37Rv]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT28092 WP_003410014.1 NC_018143:c2234899-2234651 [Mycobacterium tuberculosis H37Rv]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT28092 NC_018143:c2234899-2234651 [Mycobacterium tuberculosis H37Rv]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References