Detailed information of TA system TA028087

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2194651..2195354
• Replicon: chromosome
• Accession: NC_018143
• Organism: Mycobacterium tuberculosis H37Rv

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: RVBD_RS10190
• Protein ID: WP_003409778.1
• Coordinates: 2194651..2194980 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: RVBD_RS21335
• Protein ID: WP_003409780.1
• Coordinates: 2194977..2195354 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RVBD_RS10170	2191034..2192104	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
RVBD_RS10175	2192101..2192616	+	516	WP_003409718.1	flavin reductase family protein	-
RVBD_RS10180	2192613..2193674	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
RVBD_RS10185	2193671..2194441	+	771	WP_003409775.1	SDR family oxidoreductase	-
RVBD_RS10190	2194651..2194980	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
RVBD_RS21335	2194977..2195354	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
RVBD_RS10200	2195351..2195941	-	591	WP_003409784.1	SEC-C domain-containing protein	-
RVBD_RS10205	2195996..2197360	+	1365	WP_003903691.1	HNH endonuclease	-
RVBD_RS10210	2197515..2197967	-	453	WP_003899095.1	lipoprotein	-
RVBD_RS10215	2198031..2198432	+	402	WP_003409869.1	hypothetical protein	-
RVBD_RS10220	2198425..2198607	-	183	WP_003409870.1	hypothetical protein	-
RVBD_RS10225	2198721..2199071	-	351	WP_003899096.1	hypothetical protein	-
RVBD_RS10230	2199082..2199984	-	903	WP_003899097.1	hypothetical protein	-
RVBD_RS10235	2200005..2200196	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T28087 WP_003409778.1 NC_018143:c2194980-2194651 [Mycobacterium tuberculosis H37Rv]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T28087 NC_018143:c2194980-2194651 [Mycobacterium tuberculosis H37Rv]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT28087 WP_003409780.1 NC_018143:c2195354-2194977 [Mycobacterium tuberculosis H37Rv]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT28087 NC_018143:c2195354-2194977 [Mycobacterium tuberculosis H37Rv]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8