Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 516930..517566 Replicon chromosome
Accession NZ_CP124601
Organism Bacillus stercoris strain BS21

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag QI003_RS02610 Protein ID WP_003156187.1
Coordinates 517216..517566 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag QI003_RS02605 Protein ID WP_003225183.1
Coordinates 516930..517211 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QI003_RS02585 (QI003_02585) 513290..513889 - 600 WP_040084115.1 rhomboid family intramembrane serine protease -
QI003_RS02590 (QI003_02590) 513983..514348 + 366 WP_069839917.1 holo-ACP synthase -
QI003_RS02595 (QI003_02595) 514514..515530 + 1017 WP_080478309.1 outer membrane lipoprotein carrier protein LolA -
QI003_RS02600 (QI003_02600) 515645..516814 + 1170 WP_014662852.1 alanine racemase -
QI003_RS02605 (QI003_02605) 516930..517211 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
QI003_RS02610 (QI003_02610) 517216..517566 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
QI003_RS02615 (QI003_02615) 517681..518505 + 825 WP_014662853.1 RsbT co-antagonist protein RsbRA -
QI003_RS02620 (QI003_02620) 518510..518875 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
QI003_RS02625 (QI003_02625) 518879..519280 + 402 WP_003240352.1 serine/threonine-protein kinase RsbT -
QI003_RS02630 (QI003_02630) 519292..520299 + 1008 WP_095432087.1 phosphoserine phosphatase RsbU -
QI003_RS02635 (QI003_02635) 520361..520690 + 330 WP_014662855.1 anti-sigma factor antagonist RsbV -
QI003_RS02640 (QI003_02640) 520687..521169 + 483 WP_014662856.1 anti-sigma B factor RsbW -
QI003_RS02645 (QI003_02645) 521135..521923 + 789 WP_014662857.1 RNA polymerase sigma factor SigB -
QI003_RS02650 (QI003_02650) 521923..522522 + 600 WP_014662858.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T280869 WP_003156187.1 NZ_CP124601:517216-517566 [Bacillus stercoris]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT280869 WP_003225183.1 NZ_CP124601:516930-517211 [Bacillus stercoris]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References