Detailed information of TA system TA280865

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 3729270..3730312
• Replicon: chromosome
• Accession: NZ_CP124600
• Organism: Pseudomonas aeruginosa strain Li010

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: QIU11_RS17525
• Protein ID: WP_003153636.1
• Coordinates: 3729270..3729845 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: QIU11_RS17530
• Protein ID: WP_003050245.1
• Coordinates: 3729842..3730312 (-)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QIU11_RS17495 (QIU11_17495)	3724866..3725768	-	903	WP_003153640.1	CBASS oligonucleotide cyclase	-
QIU11_RS17500 (QIU11_17500)	3725768..3726268	-	501	WP_003090159.1	type III CBASS phage resistance system CD-NTase-associated protein Cap7	-
QIU11_RS17505 (QIU11_17505)	3726265..3726735	-	471	WP_003153638.1	type III CBASS phage resistance system CD-NTase-associated protein Cap8	-
QIU11_RS17510 (QIU11_17510)	3726732..3726989	-	258	Protein_3466	cell division protein	-
QIU11_RS17515 (QIU11_17515)	3727049..3728410	+	1362	WP_033867425.1	IS1182 family transposase	-
QIU11_RS17520 (QIU11_17520)	3728587..3729252	-	666	Protein_3468	AAA family ATPase	-
QIU11_RS17525 (QIU11_17525)	3729270..3729845	-	576	WP_003153636.1	PIN domain-containing protein	Toxin
QIU11_RS17530 (QIU11_17530)	3729842..3730312	-	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
QIU11_RS17535 (QIU11_17535)	3730516..3730899	+	384	WP_003090167.1	RAQPRD family integrative conjugative element protein	-
QIU11_RS17540 (QIU11_17540)	3730896..3731129	+	234	WP_003050225.1	TIGR03758 family integrating conjugative element protein	-
QIU11_RS17545 (QIU11_17545)	3731146..3731505	+	360	WP_003153634.1	TIGR03745 family integrating conjugative element membrane protein	-
QIU11_RS17550 (QIU11_17550)	3731518..3731916	+	399	WP_003153632.1	TIGR03750 family conjugal transfer protein	-
QIU11_RS17555 (QIU11_17555)	3731913..3732605	+	693	WP_004350603.1	TIGR03746 family integrating conjugative element protein	-
QIU11_RS17560 (QIU11_17560)	3732602..3733513	+	912	WP_004350604.1	TIGR03749 family integrating conjugative element protein	-
QIU11_RS17565 (QIU11_17565)	3733503..3734921	+	1419	WP_004350605.1	TIGR03752 family integrating conjugative element protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3675719..3784713	108994
	-	flank	IS/Tn	-	-	3727049..3728410	1361

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.78 Da
• Isoelectric Point: 5.9995

>T280865 WP_003153636.1 NZ_CP124600:c3729845-3729270 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT280865 WP_003050245.1 NZ_CP124600:c3730312-3729842 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

Structures

Antitoxin

• AlphaFold DB: I3TV68