Detailed information of TA system TA028078

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1239421..1239989
• Replicon: chromosome
• Accession: NC_018143
• Organism: Mycobacterium tuberculosis H37Rv

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: RVBD_RS05915
• Protein ID: WP_003405865.1
• Coordinates: 1239615..1239989 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: RVBD_RS05910
• Protein ID: WP_003405863.1
• Coordinates: 1239421..1239618 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RVBD_RS05890	1235462..1236100	-	639	WP_003898730.1	lipid droplet-associated protein	-
RVBD_RS05895	1236190..1237197	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
RVBD_RS05900	1237214..1238197	-	984	WP_003898731.1	hypothetical protein	-
RVBD_RS05905	1238260..1239333	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
RVBD_RS05910	1239421..1239618	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
RVBD_RS05915	1239615..1239989	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
RVBD_RS05920	1240192..1240890	+	699	WP_003898733.1	hypothetical protein	-
RVBD_RS05925	1241008..1241193	+	186	WP_003901093.1	hypothetical protein	-
RVBD_RS05930	1241120..1241395	-	276	WP_003405867.1	hypothetical protein	-
RVBD_RS05935	1241638..1241961	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
RVBD_RS05940	1241976..1242686	-	711	WP_003911399.1	hypothetical protein	-
RVBD_RS22645	1242708..1242917	+	210	WP_003911400.1	hypothetical protein	-
RVBD_RS22650	1242869..1243576	-	708	Protein_1164	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T28078 WP_003405865.1 NC_018143:1239615-1239989 [Mycobacterium tuberculosis H37Rv]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T28078 NC_018143:1239615-1239989 [Mycobacterium tuberculosis H37Rv]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT28078 WP_003405863.1 NC_018143:1239421-1239618 [Mycobacterium tuberculosis H37Rv]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT28078 NC_018143:1239421-1239618 [Mycobacterium tuberculosis H37Rv]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07