Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4153866..4154442 Replicon chromosome
Accession NZ_CP124515
Organism Escherichia coli strain AVS0282

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag QJP70_RS20275 Protein ID WP_001297643.1
Coordinates 4154155..4154442 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag QJP70_RS20270 Protein ID WP_000063156.1
Coordinates 4153866..4154168 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QJP70_RS20255 (4150462) 4150462..4152612 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
QJP70_RS20260 (4152662) 4152662..4152865 + 204 WP_000467859.1 YbdD/YjiX family protein -
QJP70_RS20265 (4152876) 4152876..4153832 + 957 WP_001297640.1 GTPase -
QJP70_RS20270 (4153866) 4153866..4154168 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
QJP70_RS20275 (4154155) 4154155..4154442 - 288 WP_001297643.1 BrnT family toxin Toxin
QJP70_RS20280 (4154641) 4154641..4155338 - 698 Protein_3979 IS1 family transposase -
QJP70_RS20285 (4155395) 4155395..4155490 + 96 Protein_3980 dienelactone hydrolase family protein -
QJP70_RS20290 (4155636) 4155636..4156868 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
QJP70_RS20295 (4156909) 4156909..4158189 + 1281 WP_001037382.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T280775 WP_001297643.1 NZ_CP124515:c4154442-4154155 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT280775 WP_000063156.1 NZ_CP124515:c4154168-4153866 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References