28077

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1230665..1231296 Replicon chromosome
Accession NC_018143
Organism Mycobacterium tuberculosis H37Rv

Toxin (Protein)


Gene name mazF Uniprot ID P9WIH8
Locus tag RVBD_RS05855 Protein ID WP_003898728.1
Coordinates 1230665..1230976 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag RVBD_RS05860 Protein ID WP_003405836.1
Coordinates 1230976..1231296 (-) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RVBD_RS05835 1226146..1227570 - 1425 WP_003405805.1 class II fumarate hydratase -
RVBD_RS05840 1227601..1228689 - 1089 WP_003898726.1 class II fructose-bisphosphatase -
RVBD_RS05845 1228745..1229389 + 645 WP_003915494.1 DUF4245 domain-containing protein -
RVBD_RS05850 1229396..1230553 - 1158 WP_003898727.1 AI-2E family transporter -
RVBD_RS05855 1230665..1230976 - 312 WP_003898728.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
RVBD_RS05860 1230976..1231296 - 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
RVBD_RS21055 1231306..1232831 + 1526 Protein_1148 carboxylesterase/lipase family protein -
RVBD_RS05875 1232849..1233961 - 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
RVBD_RS05880 1233971..1234228 - 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
RVBD_RS05885 1234218..1235465 - 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
RVBD_RS05890 1235462..1236100 - 639 WP_003898730.1 lipid droplet-associated protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11049.76 Da        Isoelectric Point: 4.9371

>T28077 WP_003898728.1 NC_018143:c1230976-1230665 [Mycobacterium tuberculosis H37Rv]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNTQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T28077 NC_018143:c1230976-1230665 [Mycobacterium tuberculosis H37Rv]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACACCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT28077 WP_003405836.1 NC_018143:c1231296-1230976 [Mycobacterium tuberculosis H37Rv]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp

>AT28077 NC_018143:c1231296-1230976 [Mycobacterium tuberculosis H37Rv]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CCA
PDB 5UCT

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References