Detailed information of TA system TA028074

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1014685..1015304
• Replicon: chromosome
• Accession: NC_018143
• Organism: Mycobacterium tuberculosis H37Rv

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: RVBD_RS04805
• Protein ID: WP_003404726.1
• Coordinates: 1014870..1015304 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: P9WJ06
• Locus tag: RVBD_RS04800
• Protein ID: WP_003898641.1
• Coordinates: 1014685..1014864 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RVBD_RS04790	1010158..1011738	+	1581	WP_003909313.1	serine hydrolase	-
RVBD_RS04795	1011735..1014128	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
RVBD_RS22400	1014401..1014559	+	159	WP_003404720.1	hypothetical protein	-
RVBD_RS04800	1014685..1014864	+	180	WP_003898641.1	antitoxin	Antitoxin
RVBD_RS04805	1014870..1015304	+	435	WP_003404726.1	SRPBCC family protein	Toxin
RVBD_RS04810	1015402..1016175	+	774	WP_003404735.1	VOC family protein	-
RVBD_RS04815	1016240..1016689	+	450	WP_003404738.1	hypothetical protein	-
RVBD_RS04820	1016824..1017054	-	231	WP_003898642.1	hypothetical protein	-
RVBD_RS04825	1017221..1018729	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
RVBD_RS04830	1018731..1019969	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T28074 WP_003404726.1 NC_018143:1014870-1015304 [Mycobacterium tuberculosis H37Rv]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T28074 NC_018143:1014870-1015304 [Mycobacterium tuberculosis H37Rv]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6403.07 Da
• Isoelectric Point: 4.5393

>AT28074 WP_003898641.1 NC_018143:1014685-1014864 [Mycobacterium tuberculosis H37Rv]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLHDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT28074 NC_018143:1014685-1014864 [Mycobacterium tuberculosis H37Rv]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCATGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Antitoxin

• AlphaFold DB: A0A045KDK5