Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 2068051..2068627 Replicon chromosome
Accession NZ_CP124511
Organism Escherichia coli strain AVS0501

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag QJP59_RS10435 Protein ID WP_001297643.1
Coordinates 2068340..2068627 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag QJP59_RS10430 Protein ID WP_000063156.1
Coordinates 2068051..2068353 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QJP59_RS10415 (2064647) 2064647..2066797 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
QJP59_RS10420 (2066847) 2066847..2067050 + 204 WP_000467859.1 YbdD/YjiX family protein -
QJP59_RS10425 (2067061) 2067061..2068017 + 957 WP_001297640.1 GTPase -
QJP59_RS10430 (2068051) 2068051..2068353 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
QJP59_RS10435 (2068340) 2068340..2068627 - 288 WP_001297643.1 BrnT family toxin Toxin
QJP59_RS10440 (2068826) 2068826..2069523 - 698 Protein_2051 IS1 family transposase -
QJP59_RS10445 (2069580) 2069580..2069675 + 96 Protein_2052 dienelactone hydrolase family protein -
QJP59_RS10450 (2069821) 2069821..2071053 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
QJP59_RS10455 (2071094) 2071094..2072374 + 1281 WP_001037382.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2068826..2069035 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T280724 WP_001297643.1 NZ_CP124511:c2068627-2068340 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT280724 WP_000063156.1 NZ_CP124511:c2068353-2068051 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References