Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4068854..4069430 Replicon chromosome
Accession NZ_CP124508
Organism Escherichia coli strain AVS0068

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag QJB02_RS19810 Protein ID WP_001297643.1
Coordinates 4069143..4069430 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag QJB02_RS19805 Protein ID WP_000063156.1
Coordinates 4068854..4069156 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QJB02_RS19790 (4065450) 4065450..4067600 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
QJB02_RS19795 (4067650) 4067650..4067853 + 204 WP_000467859.1 YbdD/YjiX family protein -
QJB02_RS19800 (4067864) 4067864..4068820 + 957 WP_001297640.1 GTPase -
QJB02_RS19805 (4068854) 4068854..4069156 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
QJB02_RS19810 (4069143) 4069143..4069430 - 288 WP_001297643.1 BrnT family toxin Toxin
QJB02_RS19815 (4069629) 4069629..4070326 - 698 Protein_3882 IS1 family transposase -
QJB02_RS19820 (4070383) 4070383..4070478 + 96 Protein_3883 dienelactone hydrolase family protein -
QJB02_RS19825 (4070624) 4070624..4071856 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
QJB02_RS19830 (4071897) 4071897..4073177 + 1281 WP_001037382.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4069629..4069838 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T280709 WP_001297643.1 NZ_CP124508:c4069430-4069143 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT280709 WP_000063156.1 NZ_CP124508:c4069156-4068854 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References