Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4141433..4142009 Replicon chromosome
Accession NZ_CP124498
Organism Escherichia coli strain AVS0655

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag QJP65_RS20255 Protein ID WP_001297643.1
Coordinates 4141722..4142009 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag QJP65_RS20250 Protein ID WP_000063156.1
Coordinates 4141433..4141735 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QJP65_RS20235 (4138029) 4138029..4140179 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
QJP65_RS20240 (4140229) 4140229..4140432 + 204 WP_000467859.1 YbdD/YjiX family protein -
QJP65_RS20245 (4140443) 4140443..4141399 + 957 WP_001297640.1 GTPase -
QJP65_RS20250 (4141433) 4141433..4141735 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
QJP65_RS20255 (4141722) 4141722..4142009 - 288 WP_001297643.1 BrnT family toxin Toxin
QJP65_RS20260 (4142208) 4142208..4142905 - 698 Protein_3976 IS1 family transposase -
QJP65_RS20265 (4142962) 4142962..4143057 + 96 Protein_3977 dienelactone hydrolase family protein -
QJP65_RS20270 (4143203) 4143203..4144435 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
QJP65_RS20275 (4144476) 4144476..4145756 + 1281 WP_001037382.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4142208..4142417 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T280619 WP_001297643.1 NZ_CP124498:c4142009-4141722 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT280619 WP_000063156.1 NZ_CP124498:c4141735-4141433 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References