Detailed information of TA system TA028061

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 640231..640907
• Replicon: chromosome
• Accession: NC_018143
• Organism: Mycobacterium tuberculosis H37Rv

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TPQ9
• Locus tag: RVBD_RS02875
• Protein ID: WP_003898500.1
• Coordinates: 640231..640644 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TPR0
• Locus tag: RVBD_RS02880
• Protein ID: WP_003402915.1
• Coordinates: 640641..640907 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RVBD_RS02845	635576..635878	-	303	WP_003402896.1	DUF3349 domain-containing protein	-
RVBD_RS02850	635938..636216	-	279	WP_003402901.1	hypothetical protein	-
RVBD_RS02855	636213..637466	-	1254	WP_003911224.1	inorganic phosphate transporter family protein	-
RVBD_RS02860	637586..637972	-	387	WP_003402907.1	VOC family protein	-
RVBD_RS02865	638035..638919	-	885	WP_003402909.1	SDR family oxidoreductase	-
RVBD_RS02870	639015..639917	-	903	WP_003915439.1	1,4-dihydroxy-2-naphthoyl-CoA synthase	-
RVBD_RS02875	640231..640644	-	414	WP_003898500.1	PIN domain-containing protein	Toxin
RVBD_RS02880	640641..640907	-	267	WP_003402915.1	antitoxin VapB3	Antitoxin
RVBD_RS02885	641099..642814	-	1716	WP_003402917.1	fatty-acid--CoA ligase FadD8	-
RVBD_RS02890	642892..644496	+	1605	WP_003402920.1	amidohydrolase	-
RVBD_RS02895	644493..645473	+	981	WP_003402923.1	o-succinylbenzoate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (13-129)

Antitoxin

• Post-segregation antitoxin CcdA (19-61)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14667.78 Da
• Isoelectric Point: 7.3603

>T28061 WP_003898500.1 NC_018143:c640644-640231 [Mycobacterium tuberculosis H37Rv]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Nucleotide

• Length: 414 bp

>T28061 NC_018143:c640644-640231 [Mycobacterium tuberculosis H37Rv]
GTGAGAGCATCGCCCACTTCGCCGCCGGAGCAGGTGGTCGTCGACGCGAGTGCCATGGTGGATCTACTGGCTCGCACTAG
CGATCGGTGCTCTGCGGTGCGCGCGCGGCTGGCTCGGACCGCGATGCACGCGCCGGCGCACTTCGATGCAGAGGTGTTGT
CGGCGCTGGGGCGCATGCAGCGCGCCGGCGCACTCACCGTTGCCTATGTCGATGCGGCACTGGAGGAGTTGCGACAGGTG
CCGGTGACTCGACACGGTCTTTCGTCGCTGCTTGCTGGAGCGTGGTCGCGCCGCGACACCCTCCGCCTGACCGATGCCCT
CTACGTCGAGCTGGCCGAAACGGCAGGTCTGGTGTTGTTGACCACCGACGAAAGATTGGCACGCGCCTGGCCCTCGGCTC
ACGCCATCGGCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9543.79 Da
• Isoelectric Point: 4.5994

>AT28061 WP_003402915.1 NC_018143:c640907-640641 [Mycobacterium tuberculosis H37Rv]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Nucleotide

• Length: 267 bp

>AT28061 NC_018143:c640907-640641 [Mycobacterium tuberculosis H37Rv]
TTGTTGAGCCGGCGCACGAAAACGATCGTTGTGTGTACATTGGTGTGTATGGCTCGGTTGAACGTGTATGTGCCCGACGA
ATTGGCGGAGCGCGCCAGGGCGCGGGGCTTGAACGTCTCGGCGCTGACTCAGGCCGCGATCAGTGCCGAGTTGGAGAACT
CCGCAACCGATGCGTGGCTTGAGGGGTTGGAACCCAGAAGCACCGGCGCTCGGCATGATGACGTGCTGGGTGCGATCGAT
GCCGCTCGCGATGAGTTCGAAGCGTGA

Structures

Toxin

• AlphaFold DB: G0TPQ9

Antitoxin

• AlphaFold DB: G0TPR0