Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4075704..4076280 Replicon chromosome
Accession NZ_CP124492
Organism Escherichia coli strain AVS0225

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag QJA99_RS19920 Protein ID WP_001297643.1
Coordinates 4075993..4076280 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag QJA99_RS19915 Protein ID WP_000063156.1
Coordinates 4075704..4076006 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QJA99_RS19900 (4072300) 4072300..4074450 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
QJA99_RS19905 (4074500) 4074500..4074703 + 204 WP_000467859.1 YbdD/YjiX family protein -
QJA99_RS19910 (4074714) 4074714..4075670 + 957 WP_001297640.1 GTPase -
QJA99_RS19915 (4075704) 4075704..4076006 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
QJA99_RS19920 (4075993) 4075993..4076280 - 288 WP_001297643.1 BrnT family toxin Toxin
QJA99_RS19925 (4076479) 4076479..4077176 - 698 Protein_3906 IS1 family transposase -
QJA99_RS19930 (4077233) 4077233..4077328 + 96 Protein_3907 dienelactone hydrolase family protein -
QJA99_RS19935 (4077474) 4077474..4078706 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
QJA99_RS19940 (4078747) 4078747..4080027 + 1281 WP_001037382.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4076479..4076688 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T280572 WP_001297643.1 NZ_CP124492:c4076280-4075993 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT280572 WP_000063156.1 NZ_CP124492:c4076006-4075704 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References