Detailed information of TA system TA028056

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 332708..333342
• Replicon: chromosome
• Accession: NC_018143
• Organism: Mycobacterium tuberculosis H37Rv

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF84
• Locus tag: RVBD_RS01460
• Protein ID: WP_003401406.1
• Coordinates: 332708..333136 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: RVBD_RS01465
• Protein ID: WP_011961607.1
• Coordinates: 333160..333342 (-)
• Length: 61 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RVBD_RS01435	328575..329708	-	1134	WP_003401394.1	hypothetical protein	-
RVBD_RS01440	329705..330325	-	621	WP_003401397.1	TetR/AcrR family transcriptional regulator	-
RVBD_RS01445	330422..331003	+	582	WP_003401399.1	VOC family protein	-
RVBD_RS01450	330933..331658	-	726	WP_003899886.1	TetR/AcrR family transcriptional regulator	-
RVBD_RS01455	331748..332668	+	921	WP_003401404.1	DUF2236 domain-containing protein	-
RVBD_RS01460	332708..333136	-	429	WP_003401406.1	type II toxin-antitoxin system VapC family toxin	Toxin
RVBD_RS01465	333160..333342	-	183	WP_011961607.1	hypothetical protein	Antitoxin
RVBD_RS01470	333437..336310	-	2874	WP_011961608.1	PE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-133)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15887.27 Da
• Isoelectric Point: 7.2214

>T28056 WP_003401406.1 NC_018143:c333136-332708 [Mycobacterium tuberculosis H37Rv]
MFLIDVNVLLAAHRGDHPNHRTVRPWFDRLLAADDPFTVPNLVWASFLRLTTNRRIFEIPSPRADAFAFVEAVNAQPHHL
PTSPGPRHLVLLRKLCDEADASGDLIPDAVLGAIAVEHHCAVVSLDRDFARFASVRHIRPPI

Nucleotide

• Length: 429 bp

>T28056 NC_018143:c333136-332708 [Mycobacterium tuberculosis H37Rv]
ATGTTCCTCATCGACGTCAACGTGCTGCTGGCTGCACACCGCGGTGACCATCCGAATCACCGAACCGTCCGCCCCTGGTT
CGATCGACTGCTTGCGGCCGACGACCCCTTCACAGTGCCGAACCTAGTGTGGGCGTCGTTCCTCCGGCTAACAACGAATC
GACGCATCTTCGAGATTCCGTCACCACGAGCGGATGCATTCGCATTCGTCGAAGCCGTCAACGCCCAGCCCCATCACCTT
CCGACAAGCCCCGGCCCCAGACACCTGGTGCTGCTGCGAAAACTCTGCGACGAGGCCGACGCATCGGGCGACTTGATACC
TGACGCGGTACTCGGGGCCATCGCCGTGGAGCATCACTGCGCCGTGGTGAGCCTGGATAGGGATTTCGCCCGGTTTGCCT
CTGTGCGCCATATTCGCCCGCCAATCTAG

Antitoxin

Protein

• Length: 61 a.a.
• Molecular weight: 6262.06 Da
• Isoelectric Point: 4.5878

>AT28056 WP_011961607.1 NC_018143:c333342-333160 [Mycobacterium tuberculosis H37Rv]
MGAVIEDALRRELAAARTGGARPTVPVFDAGTGPRPGIDLTSNTVLSEVLDEGLELNSRK

Nucleotide

• Length: 183 bp

>AT28056 NC_018143:c333342-333160 [Mycobacterium tuberculosis H37Rv]
CTGGGCGCTGTGATCGAAGACGCCCTTCGGCGGGAGCTCGCCGCCGCCCGTACCGGCGGCGCCCGGCCAACCGTCCCCGT
TTTTGACGCCGGCACCGGTCCGCGGCCAGGTATCGACCTAACCTCGAATACGGTGTTGTCCGAAGTGCTCGACGAGGGTC
TGGAACTGAACTCGCGGAAGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BRM6