Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4017834..4018410 Replicon chromosome
Accession NZ_CP124490
Organism Escherichia coli strain AVS0456

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag QJP91_RS19465 Protein ID WP_001297643.1
Coordinates 4018123..4018410 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag QJP91_RS19460 Protein ID WP_000063156.1
Coordinates 4017834..4018136 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QJP91_RS19445 (4014430) 4014430..4016580 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
QJP91_RS19450 (4016630) 4016630..4016833 + 204 WP_000467859.1 YbdD/YjiX family protein -
QJP91_RS19455 (4016844) 4016844..4017800 + 957 WP_112043124.1 GTPase -
QJP91_RS19460 (4017834) 4017834..4018136 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
QJP91_RS19465 (4018123) 4018123..4018410 - 288 WP_001297643.1 BrnT family toxin Toxin
QJP91_RS19470 (4018609) 4018609..4019306 - 698 Protein_3821 IS1 family transposase -
QJP91_RS19475 (4019363) 4019363..4019458 + 96 Protein_3822 dienelactone hydrolase family protein -
QJP91_RS19480 (4019604) 4019604..4020836 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
QJP91_RS19485 (4020877) 4020877..4022157 + 1281 WP_001037382.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4018609..4018818 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T280553 WP_001297643.1 NZ_CP124490:c4018410-4018123 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT280553 WP_000063156.1 NZ_CP124490:c4018136-4017834 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References