Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4157105..4157681 Replicon chromosome
Accession NZ_CP124487
Organism Escherichia coli strain AVS0542

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag QJP68_RS20400 Protein ID WP_001297643.1
Coordinates 4157394..4157681 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag QJP68_RS20395 Protein ID WP_000063156.1
Coordinates 4157105..4157407 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QJP68_RS20380 (4153701) 4153701..4155851 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
QJP68_RS20385 (4155901) 4155901..4156104 + 204 WP_000467859.1 YbdD/YjiX family protein -
QJP68_RS20390 (4156115) 4156115..4157071 + 957 WP_001297640.1 GTPase -
QJP68_RS20395 (4157105) 4157105..4157407 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
QJP68_RS20400 (4157394) 4157394..4157681 - 288 WP_001297643.1 BrnT family toxin Toxin
QJP68_RS20405 (4157880) 4157880..4158577 - 698 Protein_4005 IS1 family transposase -
QJP68_RS20410 (4158634) 4158634..4158729 + 96 Protein_4006 dienelactone hydrolase family protein -
QJP68_RS20415 (4158875) 4158875..4160107 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
QJP68_RS20420 (4160148) 4160148..4161428 + 1281 WP_001037382.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T280528 WP_001297643.1 NZ_CP124487:c4157681-4157394 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT280528 WP_000063156.1 NZ_CP124487:c4157407-4157105 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References