Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4251350..4251926 Replicon chromosome
Accession NZ_CP124475
Organism Escherichia coli strain AVS0969

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag QJP78_RS21210 Protein ID WP_001297643.1
Coordinates 4251639..4251926 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag QJP78_RS21205 Protein ID WP_000063156.1
Coordinates 4251350..4251652 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QJP78_RS21190 (4247946) 4247946..4250096 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
QJP78_RS21195 (4250146) 4250146..4250349 + 204 WP_000467859.1 YbdD/YjiX family protein -
QJP78_RS21200 (4250360) 4250360..4251316 + 957 WP_001297640.1 GTPase -
QJP78_RS21205 (4251350) 4251350..4251652 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
QJP78_RS21210 (4251639) 4251639..4251926 - 288 WP_001297643.1 BrnT family toxin Toxin
QJP78_RS21215 (4252125) 4252125..4252822 - 698 Protein_4155 IS1 family transposase -
QJP78_RS21220 (4252879) 4252879..4252974 + 96 Protein_4156 dienelactone hydrolase family protein -
QJP78_RS21225 (4253120) 4253120..4254352 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
QJP78_RS21230 (4254393) 4254393..4255673 + 1281 WP_001037382.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4252125..4252334 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T280442 WP_001297643.1 NZ_CP124475:c4251926-4251639 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT280442 WP_000063156.1 NZ_CP124475:c4251652-4251350 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References