Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4145491..4146067 Replicon chromosome
Accession NZ_CP124471
Organism Escherichia coli strain AVS0973

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag QJP95_RS20325 Protein ID WP_001297643.1
Coordinates 4145780..4146067 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag QJP95_RS20320 Protein ID WP_000063156.1
Coordinates 4145491..4145793 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QJP95_RS20305 (4142087) 4142087..4144237 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
QJP95_RS20310 (4144287) 4144287..4144490 + 204 WP_000467859.1 YbdD/YjiX family protein -
QJP95_RS20315 (4144501) 4144501..4145457 + 957 WP_001297640.1 GTPase -
QJP95_RS20320 (4145491) 4145491..4145793 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
QJP95_RS20325 (4145780) 4145780..4146067 - 288 WP_001297643.1 BrnT family toxin Toxin
QJP95_RS20330 (4146266) 4146266..4146963 - 698 Protein_3988 IS1 family transposase -
QJP95_RS20335 (4147020) 4147020..4147115 + 96 Protein_3989 dienelactone hydrolase family protein -
QJP95_RS20340 (4147261) 4147261..4148493 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
QJP95_RS20345 (4148534) 4148534..4149814 + 1281 WP_001037382.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4146266..4146475 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T280417 WP_001297643.1 NZ_CP124471:c4146067-4145780 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT280417 WP_000063156.1 NZ_CP124471:c4145793-4145491 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References