Detailed information of TA system TA028037

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 71994..72516
• Replicon: plasmid pKOX_R1
• Accession: NC_018107
• Organism: Klebsiella oxytoca E718

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A7H0EVH6
• Locus tag: A225_RS28895
• Protein ID: WP_014839901.1
• Coordinates: 72232..72516 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A7H0EVH5
• Locus tag: A225_RS28890
• Protein ID: WP_014839900.1
• Coordinates: 71994..72242 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A225_RS28860	67174..67530	+	357	WP_017901071.1	hypothetical protein	-
A225_RS32565	67903..68301	+	399	WP_014839899.1	H-NS histone family protein	-
A225_RS28870	68437..69045	+	609	WP_024266306.1	DUF2913 family protein	-
A225_RS28880	69589..70221	+	633	WP_001567369.1	hypothetical protein	-
A225_RS28885	70250..71653	-	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
A225_RS28890	71994..72242	+	249	WP_014839900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
A225_RS28895	72232..72516	+	285	WP_014839901.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A225_RS28900	72707..73183	+	477	WP_042934526.1	hypothetical protein	-
A225_RS28905	73189..73866	+	678	WP_042934501.1	hypothetical protein	-
A225_RS33245	73883..74602	-	720	WP_042934502.1	cation:proton antiporter	-
A225_RS28915	74843..75445	-	603	WP_014839902.1	hypothetical protein	-
A225_RS28920	75442..75962	-	521	Protein_88	hypothetical protein	-
A225_RS28925	76043..76987	-	945	WP_014839903.1	DsbA family protein	-
A225_RS34200	77050..77259	-	210	WP_125316032.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	catA2 / aac(3)-IId / mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12 / blaCTX-M-3 / blaTEM-1B / ARR-3 / aac(6')-Ib-cr / fosA3 / blaSHV-12 / sul2 / aph(3'')-Ib / aph(6)-Id / qnrS1 / dfrA1 / aadA5	-	1..353865	353865
	-	flank	IS/Tn	-	-	70250..71653	1403

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10866.46 Da
• Isoelectric Point: 10.5831

>T28037 WP_014839901.1 NC_018107:72232-72516 [Klebsiella michiganensis E718]
MTYKLAFNESALKEWKKLGHTIREQFKKKLSERLQNPRVPAAQLHGGKDQYKIKLHGAGYRLVYSVNDEIVTVTVIGVGK
RNNDDIYNATRHRN

Nucleotide

• Length: 285 bp

>T28037 NC_018107:72232-72516 [Klebsiella michiganensis E718]
ATGACCTATAAGTTAGCCTTCAATGAATCAGCTTTGAAAGAATGGAAAAAACTGGGTCATACTATTCGGGAGCAATTCAA
AAAGAAGCTTTCCGAACGGCTTCAGAACCCACGGGTGCCCGCAGCACAACTCCATGGAGGTAAAGACCAGTATAAAATAA
AGCTCCATGGCGCGGGTTACCGACTGGTTTACAGTGTCAACGATGAAATTGTGACGGTCACCGTAATTGGGGTGGGCAAA
CGCAACAATGATGATATCTACAACGCAACCCGACACAGAAATTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9071.29 Da
• Isoelectric Point: 3.8832

>AT28037 WP_014839900.1 NC_018107:71994-72242 [Klebsiella michiganensis E718]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMEMLEDQELGRIADERIGEDRIEVNID
DL

Nucleotide

• Length: 249 bp

>AT28037 NC_018107:71994-72242 [Klebsiella michiganensis E718]
ATGGCATTTCAGGTTTTAACCACCACAGCAGCCAGCATTACAGAACTTAAACGTGACCCTATGGGTACCTTCAATGCAGG
AGAAGGTGCACCGGTAGCAATCCTGAATCGCAACGAACCCGCTTTTTACTGTGTACCACCAGCCCTGTATGCTCAGCTGA
TGGAAATGCTGGAAGATCAAGAGTTGGGGCGTATTGCTGATGAACGAATCGGTGAGGACCGAATCGAGGTCAACATTGAT
GACCTATAA

Structures

Toxin

• AlphaFold DB: A0A7H0EVH6

Antitoxin

• AlphaFold DB: A0A7H0EVH5