Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3461589..3462165 Replicon chromosome
Accession NZ_CP124460
Organism Escherichia coli strain AVS0947

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag QJP79_RS16810 Protein ID WP_001295743.1
Coordinates 3461589..3461876 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag QJP79_RS16815 Protein ID WP_000063148.1
Coordinates 3461863..3462165 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QJP79_RS16795 (3457249) 3457249..3458553 - 1305 WP_000535012.1 restriction endonuclease subunit S -
QJP79_RS16800 (3458543) 3458543..3460162 - 1620 WP_001029745.1 class I SAM-dependent DNA methyltransferase -
QJP79_RS16805 (3460226) 3460226..3461392 - 1167 WP_000800831.1 restriction endonuclease -
QJP79_RS16810 (3461589) 3461589..3461876 + 288 WP_001295743.1 BrnT family toxin Toxin
QJP79_RS16815 (3461863) 3461863..3462165 + 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
QJP79_RS16820 (3462478) 3462478..3463497 - 1020 WP_000875212.1 IS110 family transposase -
QJP79_RS16825 (3463607) 3463607..3464587 - 981 WP_283201896.1 GTPase -
QJP79_RS16830 (3464598) 3464598..3464801 - 204 WP_000467859.1 YbdD/YjiX family protein -
QJP79_RS16835 (3464851) 3464851..3467001 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3462478..3463497 1019


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T280357 WP_001295743.1 NZ_CP124460:3461589-3461876 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT280357 WP_000063148.1 NZ_CP124460:3461863-3462165 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References