Detailed information of TA system TA028035

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 5976863..5977410
• Replicon: chromosome
• Accession: NC_018106
• Organism: Klebsiella oxytoca E718

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: A225_RS27915
• Protein ID: WP_014839830.1
• Coordinates: 5977102..5977410 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A0H3H0P7
• Locus tag: A225_RS27910
• Protein ID: WP_014227298.1
• Coordinates: 5976863..5977099 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A225_RS27895	5973070..5974236	+	1167	WP_014839828.1	multidrug effflux MFS transporter	-
A225_RS27900	5974397..5975299	+	903	WP_009654080.1	N-acetylmuramic acid 6-phosphate etherase	-
A225_RS27905	5975324..5976757	+	1434	WP_014839829.1	PTS N-acetylmuramic acid transporter subunit IIBC	-
A225_RS27910	5976863..5977099	+	237	WP_014227298.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
A225_RS27915	5977102..5977410	+	309	WP_014839830.1	CcdB family protein	Toxin
A225_RS27920	5977561..5978463	+	903	WP_014227300.1	EamA family transporter	-
A225_RS27925	5978467..5978724	-	258	WP_038422923.1	hypothetical protein	-
A225_RS27930	5978724..5979689	-	966	WP_014839832.1	Rpn family recombination-promoting nuclease/putative transposase	-
A225_RS27935	5979899..5980192	+	294	WP_014227302.1	hypothetical protein	-
A225_RS27940	5980216..5981157	-	942	WP_014227303.1	LacI family transcriptional regulator	-
A225_RS27945	5981452..5981763	+	312	WP_014839834.1	PTS sugar transporter subunit IIB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (8-78)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11724.63 Da
• Isoelectric Point: 8.8012

>T28035 WP_014839830.1 NC_018106:5977102-5977410 [Klebsiella michiganensis E718]
MQYYVYKNTGRIAAYPYLLDVQSDIIGKRNTRVVIPLFPLKNYKGPRADRLTPLVTVEGEEYVVMTHELASIPHRVLGKE
VCNLNHQREVVKASMDFLFDGI

Nucleotide

• Length: 309 bp

>T28035 NC_018106:5977102-5977410 [Klebsiella michiganensis E718]
ATGCAGTATTACGTTTATAAGAATACAGGTCGAATTGCTGCGTATCCTTACCTCCTTGATGTACAGAGTGACATTATCGG
CAAGCGCAATACTCGGGTCGTGATTCCACTGTTTCCACTGAAAAACTATAAAGGCCCACGCGCCGATCGTTTAACACCGC
TTGTCACCGTAGAAGGGGAAGAATACGTCGTGATGACTCACGAACTCGCCAGCATCCCACATCGTGTTCTGGGTAAAGAA
GTTTGTAATCTGAATCACCAGCGCGAAGTCGTTAAAGCTTCAATGGATTTTCTTTTCGACGGGATCTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8783.92 Da
• Isoelectric Point: 5.7928

>AT28035 WP_014227298.1 NC_018106:5976863-5977099 [Klebsiella michiganensis E718]
MPAAKRNKQNVSVTLDPGLVKQAREVGINLSLTLTNALKSEIHSLEIARWQKENAQAIDYLNNLTEENGLLSDEYRTF

Nucleotide

• Length: 237 bp

>AT28035 NC_018106:5976863-5977099 [Klebsiella michiganensis E718]
ATGCCAGCCGCAAAACGCAATAAGCAGAACGTCAGCGTGACACTGGACCCAGGCCTGGTCAAACAGGCTCGTGAGGTCGG
CATCAATCTGTCTCTAACGTTGACTAACGCTCTAAAAAGCGAAATCCACAGCCTCGAAATAGCCCGCTGGCAAAAAGAAA
ATGCCCAGGCAATCGACTACCTCAATAATTTAACTGAGGAAAATGGACTTCTCTCTGATGAATACAGGACGTTCTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3H0P7