Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 43770..44295 Replicon plasmid pAVS0193-A
Accession NZ_CP124456
Organism Escherichia coli strain AVS0193

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag QJP76_RS25640 Protein ID WP_001159868.1
Coordinates 43990..44295 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag QJP76_RS25635 Protein ID WP_000813634.1
Coordinates 43770..43988 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QJP76_RS25605 (39183) 39183..39689 - 507 WP_000949452.1 protein disulfide oxidoreductase -
QJP76_RS25610 (39679) 39679..39849 - 171 Protein_42 DsbA family protein -
QJP76_RS25615 (39987) 39987..40099 - 113 Protein_43 IS6 family transposase -
QJP76_RS25620 (40299) 40299..40994 + 696 Protein_44 IS1 family transposase -
QJP76_RS25625 (41131) 41131..42024 - 894 WP_001553853.1 S-4TM family putative pore-forming effector -
QJP76_RS25630 (42067) 42067..43062 - 996 WP_000246636.1 hypothetical protein -
QJP76_RS25635 (43770) 43770..43988 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
QJP76_RS25640 (43990) 43990..44295 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
QJP76_RS25645 (44296) 44296..45102 + 807 WP_000016982.1 site-specific integrase -
QJP76_RS25650 (45876) 45876..46631 + 756 WP_000852146.1 replication initiation protein RepE -
QJP76_RS25655 (47219) 47219..48385 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5 / dfrA17 senB 1..146449 146449
- flank IS/Tn - - 40618..40974 356


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T280337 WP_001159868.1 NZ_CP124456:43990-44295 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT280337 WP_000813634.1 NZ_CP124456:43770-43988 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References