Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3663537..3664113 Replicon chromosome
Accession NZ_CP124436
Organism Escherichia coli strain AVS0790

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag QJP66_RS18040 Protein ID WP_001295743.1
Coordinates 3663537..3663824 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag QJP66_RS18045 Protein ID WP_000063148.1
Coordinates 3663811..3664113 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QJP66_RS18025 (3659197) 3659197..3660501 - 1305 WP_000535012.1 restriction endonuclease subunit S -
QJP66_RS18030 (3660491) 3660491..3662110 - 1620 WP_001029745.1 class I SAM-dependent DNA methyltransferase -
QJP66_RS18035 (3662174) 3662174..3663340 - 1167 WP_000800831.1 restriction endonuclease -
QJP66_RS18040 (3663537) 3663537..3663824 + 288 WP_001295743.1 BrnT family toxin Toxin
QJP66_RS18045 (3663811) 3663811..3664113 + 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
QJP66_RS18050 (3664147) 3664147..3665103 - 957 WP_001295745.1 GTPase -
QJP66_RS18055 (3665114) 3665114..3665317 - 204 WP_000467859.1 YbdD/YjiX family protein -
QJP66_RS18060 (3665367) 3665367..3667517 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T280266 WP_001295743.1 NZ_CP124436:3663537-3663824 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT280266 WP_000063148.1 NZ_CP124436:3663811-3664113 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References