Detailed information of TA system TA028023

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 707289..707865
• Replicon: chromosome
• Accession: NC_018106
• Organism: Klebsiella oxytoca E718

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A1D8JNH2
• Locus tag: A225_RS03285
• Protein ID: WP_042933975.1
• Coordinates: 707289..707576 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: A225_RS03290
• Protein ID: WP_014837314.1
• Coordinates: 707563..707865 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A225_RS03255	702622..703140	-	519	WP_014837307.1	hypothetical protein	-
A225_RS03260	703137..703937	-	801	WP_014837308.1	winged helix-turn-helix domain-containing protein	-
A225_RS03265	704083..704667	-	585	WP_004098242.1	TetR/AcrR family transcriptional regulator	-
A225_RS03270	704815..705597	-	783	WP_014837310.1	NAD(P)H-dependent oxidoreductase	-
A225_RS03275	705685..706593	+	909	WP_014837311.1	LysR family transcriptional regulator	-
A225_RS03280	706587..707030	+	444	WP_014837312.1	FosA family fosfomycin resistance glutathione transferase	-
A225_RS03285	707289..707576	+	288	WP_042933975.1	BrnT family toxin	Toxin
A225_RS03290	707563..707865	+	303	WP_014837314.1	BrnA antitoxin family protein	Antitoxin
A225_RS03295	707986..709329	-	1344	WP_004128506.1	APC family permease	-
A225_RS03300	709326..710237	-	912	WP_014837315.1	acetamidase/formamidase family protein	-
A225_RS03305	710690..711025	-	336	WP_014837316.1	endoribonuclease SymE	-
A225_RS03310	711376..712383	-	1008	Protein_638	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (39-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11239.69 Da
• Isoelectric Point: 8.5899

>T28023 WP_042933975.1 NC_018106:707289-707576 [Klebsiella michiganensis E718]
MPMEFEWDANKARSNLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTIGLVHGVIVIMVAHSVRFESGTEVIRIIS
ARKADSKERNRYEHG

Nucleotide

• Length: 288 bp

>T28023 NC_018106:707289-707576 [Klebsiella michiganensis E718]
ATGCCAATGGAGTTTGAATGGGATGCGAACAAGGCAAGAAGTAATCTAAGAAAACATGGAATCCGGTTTGAAGAAGCGGT
GCTCGTCTTTGACGATCCCCGGCATCTTTCCCGACAGGATCGTTATGAGAATGGTGAATATCGCTGGCAAACTATCGGTC
TCGTTCATGGGGTCATTGTTATCATGGTTGCGCATTCGGTCCGTTTTGAAAGTGGAACAGAGGTCATTCGTATTATCAGC
GCCCGGAAGGCGGACAGCAAAGAGAGAAATCGTTATGAACATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11549.14 Da
• Isoelectric Point: 9.7962

>AT28023 WP_014837314.1 NC_018106:707563-707865 [Klebsiella michiganensis E718]
MNMVKHKRDNPDTLTAKRDDELKALANKTDDEIDYSGIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Nucleotide

• Length: 303 bp

>AT28023 NC_018106:707563-707865 [Klebsiella michiganensis E718]
ATGAACATGGTTAAACATAAACGTGACAATCCGGATACGCTTACGGCTAAGCGAGACGATGAGCTTAAAGCGCTGGCCAA
CAAAACGGATGATGAAATCGATTATAGCGGTATTCCCGCCACAGACGACGCACAGTGGTCAGATGCCGTACGCGGTAAAT
TTTACCGACCGCTAAAAACTCAGGCTTCTGTCCGTATTGATGCCGATATTATGGAGTGGCTTAAACGTCCCGGGAAAGGT
TATCAAACGCGACTGAATGCGATCTTAAGAGAAGCGATGATTCGCGATCTTAACAAGAAATAG

Structures

Toxin

• AlphaFold DB: A0A1D8JNH2