Detailed information of TA system TA028022

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 650862..651666
• Replicon: chromosome
• Accession: NC_018106
• Organism: Klebsiella oxytoca E718

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A486SQP3
• Locus tag: A225_RS03025
• Protein ID: WP_040217122.1
• Coordinates: 651277..651666 (+)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7H4M120
• Locus tag: A225_RS03020
• Protein ID: WP_014837280.1
• Coordinates: 650862..651221 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A225_RS31210	647338..647970	-	633	WP_071886307.1	inovirus Gp2 family protein	-
A225_RS02995	648510..649334	+	825	WP_040217125.1	DUF945 domain-containing protein	-
A225_RS03000	649340..649588	+	249	WP_044347584.1	DUF905 domain-containing protein	-
A225_RS03005	649668..650111	+	444	WP_014837277.1	antirestriction protein	-
A225_RS03010	650123..650602	+	480	WP_014837278.1	DNA repair protein RadC	-
A225_RS03015	650616..650837	+	222	WP_014837279.1	DUF987 domain-containing protein	-
A225_RS03020	650862..651221	+	360	WP_014837280.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
A225_RS03025	651277..651666	+	390	WP_040217122.1	TA system toxin CbtA family protein	Toxin
A225_RS03030	651753..652598	+	846	WP_014837282.1	DUF4942 domain-containing protein	-
A225_RS03035	652740..653537	+	798	WP_014837283.1	helix-turn-helix transcriptional regulator	-
A225_RS03040	653715..654458	-	744	WP_014837284.1	hypothetical protein	-
A225_RS03045	654624..655058	-	435	WP_004098173.1	VOC family protein	-
A225_RS03050	655217..655540	-	324	WP_014837285.1	endoribonuclease SymE	-
A225_RS03055	655830..656624	-	795	WP_042933970.1	DNA/RNA non-specific endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	595426..655540	60114

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (3-111)

Antitoxin

• Antitoxin CbeA (16-114)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14477.57 Da
• Isoelectric Point: 8.9843

>T28022 WP_040217122.1 NC_018106:651277-651666 [Klebsiella michiganensis E718]
MQTKSPPFMRAASSRPSPVGVWQTLLTCLLEHHYGLTLNDTPFSDELVIQQHIEAGISLADALNFIVEKYELVRTDRPGF
SIREQSPFITSIDILRARKATGLMNRDTYKEVTAITRGQHPQVSAPGKR

Nucleotide

• Length: 390 bp

>T28022 NC_018106:651277-651666 [Klebsiella michiganensis E718]
ATGCAAACCAAATCACCCCCCTTCATGCGGGCGGCATCGTCACGTCCGTCTCCCGTGGGCGTCTGGCAGACCCTGCTGAC
CTGCCTGCTGGAACACCACTATGGCCTTACGCTTAACGATACGCCGTTCAGTGATGAACTGGTGATTCAGCAACATATCG
AGGCGGGTATCTCGCTGGCCGATGCCCTGAATTTTATTGTTGAAAAGTATGAACTGGTCCGCACTGACCGGCCAGGCTTC
AGCATCAGGGAGCAGTCTCCGTTTATTACATCCATTGATATCCTCCGGGCCAGAAAGGCAACGGGGCTGATGAACCGTGA
CACGTATAAAGAGGTGACGGCTATTACCAGAGGGCAACATCCGCAGGTCAGTGCTCCAGGCAAGCGATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13416.24 Da
• Isoelectric Point: 7.0268

>AT28022 WP_014837280.1 NC_018106:650862-651221 [Klebsiella michiganensis E718]
MSKKTTIATHDISESWWGLRRSVSACFGARLVQEGNRLHYLADRANFNGQFCDADLRHLDQSFPVLMKQLELMLTSGELN
PRHQHCVTLYAKGLTCEADTLGSHGYVYIAIYPTPVTTE

Nucleotide

• Length: 360 bp

>AT28022 NC_018106:650862-651221 [Klebsiella michiganensis E718]
TTGTCGAAGAAAACCACGATAGCAACGCATGATATCAGCGAATCCTGGTGGGGGCTCAGACGCAGTGTCTCCGCGTGTTT
TGGTGCCCGACTGGTCCAGGAGGGAAACCGCCTGCATTACCTGGCTGACCGCGCGAATTTTAACGGACAGTTCTGCGATG
CGGATTTGCGCCATCTTGACCAGTCTTTCCCGGTATTGATGAAACAGCTGGAGTTAATGCTCACCAGCGGTGAACTGAAT
CCCCGTCATCAGCACTGCGTCACGCTGTATGCAAAAGGTCTGACCTGCGAGGCCGATACGCTCGGTTCGCATGGCTACGT
TTATATCGCGATTTATCCCACTCCCGTCACCACAGAATAA

Structures

Toxin

• AlphaFold DB: A0A486SQP3

Antitoxin

• AlphaFold DB: A0A7H4M120