Detailed information of TA system TA028021

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-AbrB
• Location: 397682..398298
• Replicon: chromosome
• Accession: NC_018092
• Organism: Pyrococcus furiosus COM1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: I6U642
• Locus tag: PFC_RS02005
• Protein ID: WP_011011692.1
• Coordinates: 397897..398298 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: I6UNL1
• Locus tag: PFC_RS02000
• Protein ID: WP_011011691.1
• Coordinates: 397682..397909 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PFC_RS11110 (PFC_01970)	393483..393899	-	417	WP_014835169.1	DUF1156 domain-containing protein	-
PFC_RS01985 (PFC_01975)	393959..394384	+	426	WP_011011687.1	DUF365 domain-containing protein	-
PFC_RS11115	394365..394781	-	417	WP_011011688.1	hypothetical protein	-
PFC_RS11120	394808..395524	-	717	WP_223209002.1	hypothetical protein	-
PFC_RS01995 (PFC_01990)	395638..397605	+	1968	WP_014835170.1	IGHMBP2 family helicase	-
PFC_RS02000 (PFC_01995)	397682..397909	+	228	WP_011011691.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
PFC_RS02005 (PFC_02000)	397897..398298	+	402	WP_011011692.1	PIN domain-containing protein	Toxin
PFC_RS11125	398445..398540	-	96	WP_237718490.1	hypothetical protein	-
PFC_RS02010 (PFC_02005)	398757..399095	-	339	WP_011011693.1	PIN domain-containing protein	-
PFC_RS02015 (PFC_02010)	399082..399274	-	193	Protein_399	DUF104 domain-containing protein	-
PFC_RS02020 (PFC_02015)	399385..400236	+	852	WP_011011695.1	ABC transporter ATP-binding protein	-
PFC_RS02025 (PFC_02020)	400233..400934	+	702	WP_011011696.1	hypothetical protein	-
PFC_RS02030 (PFC_02025)	400931..401623	+	693	WP_011011697.1	permease	-
PFC_RS02035 (PFC_02030)	401884..402885	+	1002	WP_011011698.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-125)

Antitoxin

• SpoVT-AbrB domain (5-48)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 15632.01 Da
• Isoelectric Point: 6.4890

>T28021 WP_011011692.1 NC_018092:397897-398298 [Pyrococcus furiosus COM1]
MHAVIDTNVLIYDTFSDSEFHKESRSLLNSLDRWYIPSIVLQEYVWFFRSQGFSSREAKIMLSEYISDPRFRGLVEDHNV
ILRAIDILERENLSLSRFNDMIILVHAIEKGTLATFDQKLRKLARKLSVEILP

Nucleotide

• Length: 402 bp

>T28021 NC_018092:397897-398298 [Pyrococcus furiosus COM1]
ATGCATGCAGTGATAGATACAAATGTTCTAATTTATGATACATTTAGCGATTCTGAGTTTCACAAAGAGTCTAGATCTCT
TTTGAATTCTCTTGATAGATGGTACATTCCTTCCATAGTACTTCAGGAGTATGTGTGGTTTTTTAGAAGTCAAGGATTTT
CAAGTAGAGAGGCCAAGATAATGCTCTCTGAATATATATCAGATCCAAGATTCAGAGGTCTTGTAGAAGACCACAATGTA
ATTCTTCGTGCCATTGACATTCTCGAAAGGGAAAACCTCTCTTTATCACGGTTTAATGATATGATAATTCTTGTTCATGC
AATAGAGAAGGGTACCCTAGCAACTTTTGATCAAAAACTCAGAAAACTTGCAAGGAAGTTGAGCGTTGAAATCTTGCCAT
AG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8705.47 Da
• Isoelectric Point: 10.2059

>AT28021 WP_011011691.1 NC_018092:397682-397909 [Pyrococcus furiosus COM1]
MPITKVTRNYQITIPAEIRKALGIKEGELLEVRLENGKIIIERLKKERKTLKLGKKLTLEEIEKAIEEGMKQCMQ

Nucleotide

• Length: 228 bp

>AT28021 NC_018092:397682-397909 [Pyrococcus furiosus COM1]
ATGCCCATTACGAAGGTTACTAGAAACTATCAGATAACAATTCCTGCAGAGATTAGAAAGGCCCTTGGAATTAAAGAAGG
TGAACTGCTTGAAGTAAGGCTAGAAAATGGAAAAATAATTATAGAAAGACTCAAAAAAGAAAGAAAAACACTCAAGCTGG
GTAAAAAGTTAACACTCGAAGAAATTGAGAAAGCGATAGAGGAGGGAATGAAACAATGCATGCAGTGA

Structures

Toxin

• AlphaFold DB: I6U642

Antitoxin

• AlphaFold DB: I6UNL1