Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 557737..558308 Replicon chromosome
Accession NC_018081
Organism Enterococcus hirae ATCC 9790

Toxin (Protein)


Gene name mazF Uniprot ID I6SW09
Locus tag EHR_RS02790 Protein ID WP_010738536.1
Coordinates 557967..558308 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I6T4M0
Locus tag EHR_RS02785 Protein ID WP_010738535.1
Coordinates 557737..557967 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EHR_RS02775 (EHR_03045) 554754..555416 + 663 WP_014834300.1 AraC family ligand binding domain-containing protein -
EHR_RS14390 (EHR_03050) 555512..555718 + 207 WP_243464876.1 AraC family transcriptional regulator -
EHR_RS02780 (EHR_03055) 555898..557277 - 1380 WP_010738534.1 FAD-dependent oxidoreductase -
EHR_RS02785 (EHR_03060) 557737..557967 + 231 WP_010738535.1 hypothetical protein Antitoxin
EHR_RS02790 (EHR_03065) 557967..558308 + 342 WP_010738536.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
EHR_RS02795 (EHR_03070) 558472..559638 - 1167 WP_010738537.1 pyridoxal phosphate-dependent aminotransferase -
EHR_RS02800 (EHR_03075) 559668..560777 - 1110 WP_025480561.1 M20 family metallopeptidase -
EHR_RS02805 (EHR_03080) 560737..562086 - 1350 WP_010720195.1 aspartate kinase -
EHR_RS02810 (EHR_03085) 562083..563084 - 1002 WP_014834302.1 aspartate-semialdehyde dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13101.46 Da        Isoelectric Point: 10.1962

>T28017 WP_010738536.1 NC_018081:557967-558308 [Enterococcus hirae ATCC 9790]
VPKARNYIPKKGDIVWIDFDPSTGKEIQKRRPGLVVSRYEFNCTTMFAIICPITSTIKNLPTRYSLPKDLDTKGQVLISQ
LKSLDFKERKLKKVENLPLQDMAKIDQIIQYIF

Download         Length: 342 bp

>T28017 NC_018081:557967-558308 [Enterococcus hirae ATCC 9790]
GTGCCTAAAGCTAGAAATTATATTCCTAAAAAAGGGGATATTGTTTGGATCGATTTTGATCCATCCACAGGCAAAGAAAT
TCAAAAGAGAAGACCAGGCTTGGTTGTATCTCGTTATGAATTTAATTGCACAACAATGTTTGCAATAATCTGTCCAATTA
CTTCTACTATAAAAAATCTACCGACACGCTATTCGCTTCCCAAAGATTTAGATACAAAGGGACAAGTACTTATTTCTCAA
TTAAAATCACTTGATTTCAAGGAAAGAAAACTCAAAAAGGTCGAAAATTTACCTTTACAAGATATGGCTAAAATTGATCA
GATTATCCAGTATATATTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8577.57 Da        Isoelectric Point: 4.1196

>AT28017 WP_010738535.1 NC_018081:557737-557967 [Enterococcus hirae ATCC 9790]
MLITKSRLQGSSVVITLPSDNGKKPSENQEYIVVYSEDGTITLVPKIEDPFSAGQEAEYYEKDEWKDLTPEGREIL

Download         Length: 231 bp

>AT28017 NC_018081:557737-557967 [Enterococcus hirae ATCC 9790]
ATGCTCATTACAAAATCTAGATTACAAGGTAGTTCTGTAGTAATTACACTACCGTCTGATAATGGTAAAAAACCTTCAGA
AAATCAAGAATATATTGTTGTCTACTCTGAAGATGGGACAATCACATTAGTTCCTAAAATCGAAGATCCATTTAGTGCTG
GACAAGAAGCTGAGTATTACGAAAAAGATGAATGGAAAGACTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7Z9DJ27

Antitoxin

Source ID Structure
AlphaFold DB A0A449E900

References