Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataT-KacA/DUF1778(antitoxin)
Location 32261..33059 Replicon plasmid pAVS0223-B
Accession NZ_CP124417
Organism Escherichia coli strain AVS0223

Toxin (Protein)


Gene name ataT Uniprot ID E2QDF3
Locus tag QJP60_RS25155 Protein ID WP_000072677.1
Coordinates 32538..33059 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A0V9R6Q7
Locus tag QJP60_RS25150 Protein ID WP_001351987.1
Coordinates 32261..32530 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QJP60_RS25125 (QJP60_25125) 27688..29028 - 1341 WP_077136336.1 DnaB-like helicase C-terminal domain-containing protein -
QJP60_RS25130 (QJP60_25130) 29072..29812 - 741 WP_001717320.1 hypothetical protein -
QJP60_RS25135 (QJP60_25135) 30095..30862 + 768 WP_283187938.1 hypothetical protein -
QJP60_RS25140 (QJP60_25140) 30915..31268 - 354 WP_160378290.1 hypothetical protein -
QJP60_RS25145 (QJP60_25145) 31274..31942 - 669 WP_000161228.1 division plane positioning ATPase MipZ -
QJP60_RS25150 (QJP60_25150) 32261..32530 + 270 WP_001351987.1 DUF1778 domain-containing protein Antitoxin
QJP60_RS25155 (QJP60_25155) 32538..33059 + 522 WP_000072677.1 GNAT family N-acetyltransferase Toxin
QJP60_RS25160 (QJP60_25160) 33228..33479 - 252 WP_000901559.1 hypothetical protein -
QJP60_RS25165 (QJP60_25165) 33481..34173 - 693 WP_000856757.1 hypothetical protein -
QJP60_RS25170 (QJP60_25170) 34187..34510 - 324 WP_001717323.1 hypothetical protein -
QJP60_RS25175 (QJP60_25175) 34713..37379 - 2667 WP_238064378.1 phage tail protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..108564 108564


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19392.17 Da        Isoelectric Point: 8.6369

>T280146 WP_000072677.1 NZ_CP124417:32538-33059 [Escherichia coli]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLANHLKRQHEGKILRAYVLCTQEERPKVLGYYTLSGSCFEKESLPSRSKQ
KKVPYRNVPSVTLGRLALDKSLQGQGFGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKAKAFYKSLGFIQLVGNNERSL
FYPTKSIEKLFEE

Download         Length: 522 bp

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10082.54 Da        Isoelectric Point: 7.0155

>AT280146 WP_001351987.1 NZ_CP124417:32261-32530 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLNEESWNLVMDAISNPPAPNDKLKR
AADRLKSME

Download         Length: 270 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9R6P3

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9R6Q7

References