Detailed information of TA system TA028013

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2544383..2545425
• Replicon: chromosome
• Accession: NC_018080
• Organism: Pseudomonas aeruginosa DK2

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: PADK2_RS12080
• Protein ID: WP_003109777.1
• Coordinates: 2544850..2545425 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: PADK2_RS12075
• Protein ID: WP_003050245.1
• Coordinates: 2544383..2544853 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PADK2_RS12040	2539775..2541193	-	1419	WP_003116808.1	TIGR03752 family integrating conjugative element protein	-
PADK2_RS12045	2541183..2542094	-	912	WP_003116809.1	TIGR03749 family integrating conjugative element protein	-
PADK2_RS12050	2542091..2542783	-	693	WP_003116810.1	TIGR03746 family integrating conjugative element protein	-
PADK2_RS12055	2542780..2543178	-	399	WP_003050133.1	TIGR03750 family conjugal transfer protein	-
PADK2_RS12060	2543190..2543549	-	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
PADK2_RS12065	2543566..2543799	-	234	WP_003050225.1	TIGR03758 family integrating conjugative element protein	-
PADK2_RS12070	2543796..2544179	-	384	WP_003116811.1	RAQPRD family integrative conjugative element protein	-
PADK2_RS12075	2544383..2544853	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
PADK2_RS12080	2544850..2545425	+	576	WP_003109777.1	PIN domain-containing protein	Toxin
PADK2_RS12085	2545443..2546357	+	915	WP_003105629.1	AAA family ATPase	-
PADK2_RS12090	2546354..2546824	+	471	WP_003105626.1	hypothetical protein	-
PADK2_RS12095	2546821..2547321	+	501	WP_003090159.1	hypothetical protein	-
PADK2_RS12100	2547321..2548223	+	903	WP_003105624.1	nucleotidyltransferase domain-containing protein	-
PADK2_RS12105	2548262..2548987	+	726	WP_003050273.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2495259..2557712	62453

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21644.79 Da
• Isoelectric Point: 5.6172

>T28013 WP_003109777.1 NC_018080:2544850-2545425 [Pseudomonas aeruginosa DK2]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPDDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIEVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T28013 NC_018080:2544850-2545425 [Pseudomonas aeruginosa DK2]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACCGGGCAATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTGCCCGATCCGGACGACCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGGGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTACTGGATCTGGACGCGGCCGCCGTGGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGAGGTGGACCGCTATCTCGAAATCCTGCTGCGCCAGGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT28013 WP_003050245.1 NC_018080:2544383-2544853 [Pseudomonas aeruginosa DK2]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT28013 NC_018080:2544383-2544853 [Pseudomonas aeruginosa DK2]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTGAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTCGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68