Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1159516..1160136 Replicon chromosome
Accession NC_018079
Organism Enterobacter cloacae subsp. dissolvens SDM

Toxin (Protein)


Gene name Hha Uniprot ID A0A5E1A1U8
Locus tag A3UG_RS05380 Protein ID WP_013095889.1
Coordinates 1159516..1159734 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag A3UG_RS05385 Protein ID WP_008499288.1
Coordinates 1159762..1160136 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A3UG_RS05350 1155528..1155788 + 261 WP_010428154.1 type B 50S ribosomal protein L31 -
A3UG_RS05355 1155791..1155931 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
A3UG_RS05360 1155928..1156638 - 711 WP_014830898.1 GNAT family N-acetyltransferase -
A3UG_RS05365 1156740..1158200 + 1461 WP_014830899.1 PLP-dependent aminotransferase family protein -
A3UG_RS05370 1158172..1158639 - 468 WP_014830900.1 membrane protein -
A3UG_RS05375 1158755..1159306 - 552 WP_014830901.1 maltose O-acetyltransferase -
A3UG_RS05380 1159516..1159734 - 219 WP_013095889.1 hemolysin expression modulator Hha Toxin
A3UG_RS05385 1159762..1160136 - 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
A3UG_RS05390 1160649..1163795 - 3147 WP_014830902.1 multidrug efflux RND transporter permease subunit -
A3UG_RS05395 1163818..1165011 - 1194 WP_014830903.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8625.98 Da        Isoelectric Point: 8.9008

>T27999 WP_013095889.1 NC_018079:c1159734-1159516 [Enterobacter cloacae subsp. dissolvens SDM]
MSDKPLTKTDYLMRLRRCQSIDTLERVIEKNKYELSDNELTVFYSAADHRLAELTMNKLYDKIPPSVWKFVR

Download         Length: 219 bp

>T27999 NC_018079:c1159734-1159516 [Enterobacter cloacae subsp. dissolvens SDM]
ATGTCTGATAAACCACTCACAAAAACCGATTATTTGATGCGCTTACGACGCTGTCAATCAATTGACACGCTCGAACGTGT
TATTGAAAAAAATAAATACGAGCTTTCTGATAATGAACTGACTGTATTTTATTCTGCGGCGGACCACCGTCTTGCTGAGC
TGACCATGAATAAACTGTATGACAAAATCCCCCCTTCCGTGTGGAAATTTGTCCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT27999 WP_008499288.1 NC_018079:c1160136-1159762 [Enterobacter cloacae subsp. dissolvens SDM]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT27999 NC_018079:c1160136-1159762 [Enterobacter cloacae subsp. dissolvens SDM]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAATCATGGCTGGGTGAACGATCCAACGTCTGCCATCAATTTACAATTGAATGAGTTGATAGAGC
ATATCGCGACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTGATTGAGCAAATTGACGAATACCTG
GATGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCTGGAAACCGGCT
ATTCCGTTGTTTCGTCAACGTGAGCAGAGCTAACCCGGTTAGCCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1A1U8

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References