Detailed information of TA system TA027994

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3706812..3707486
• Replicon: chromosome
• Accession: NC_018078
• Organism: Mycobacterium tuberculosis KZN 605

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF52
• Locus tag: TBXG_RS17550
• Protein ID: WP_003417282.1
• Coordinates: 3706812..3707240 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TI59
• Locus tag: TBXG_RS17555
• Protein ID: WP_003417286.1
• Coordinates: 3707244..3707486 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBXG_RS17525	3702634..3703035	-	402	WP_003417264.1	cytidine deaminase	-
TBXG_RS17530	3703176..3703610	+	435	WP_003900017.1	succinate dehydrogenase, cytochrome b556 subunit	-
TBXG_RS17535	3703607..3704041	+	435	WP_003417269.1	succinate dehydrogenase hydrophobic membrane anchor subunit	-
TBXG_RS17540	3704170..3705942	+	1773	WP_003417273.1	succinate dehydrogenase flavoprotein subunit	-
TBXG_RS17545	3705942..3706733	+	792	WP_003417279.1	succinate dehydrogenase iron-sulfur subunit	-
TBXG_RS17550	3706812..3707240	-	429	WP_003417282.1	type II toxin-antitoxin system VapC family toxin	Toxin
TBXG_RS17555	3707244..3707486	-	243	WP_003417286.1	ribbon-helix-helix domain-containing protein	Antitoxin
TBXG_RS17560	3707608..3708222	-	615	WP_003417288.1	class I SAM-dependent methyltransferase	-
TBXG_RS17565	3708219..3708884	-	666	WP_003417290.1	molybdopterin converting factor subunit 1	-
TBXG_RS17570	3708885..3709418	-	534	WP_003417293.1	cyclic pyranopterin monophosphate synthase MoaC	-
TBXG_RS17575	3709415..3709789	-	375	WP_003417295.1	4a-hydroxytetrahydrobiopterin dehydratase	-
TBXG_RS17590	3711163..3711354	-	192	Protein_3440	RNA polymerase sigma factor SigJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-141)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15834.16 Da
• Isoelectric Point: 8.5471

>T27994 WP_003417282.1 NC_018078:c3707240-3706812 [Mycobacterium tuberculosis KZN 605]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Nucleotide

• Length: 429 bp

>T27994 NC_018078:c3707240-3706812 [Mycobacterium tuberculosis KZN 605]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8849.86 Da
• Isoelectric Point: 5.1007

>AT27994 WP_003417286.1 NC_018078:c3707486-3707244 [Mycobacterium tuberculosis KZN 605]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Nucleotide

• Length: 243 bp

>AT27994 NC_018078:c3707486-3707244 [Mycobacterium tuberculosis KZN 605]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBL0

Antitoxin

• AlphaFold DB: G0TI59