Detailed information of TA system TA027992

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 3396431..3397050
• Replicon: chromosome
• Accession: NC_018078
• Organism: Mycobacterium tuberculosis KZN 605

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: TBXG_RS16060
• Protein ID: WP_003404726.1
• Coordinates: 3396431..3396865 (-)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: P9WJ06
• Locus tag: TBXG_RS16065
• Protein ID: WP_003898641.1
• Coordinates: 3396871..3397050 (-)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBXG_RS16035	3391766..3393004	+	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-
TBXG_RS16040	3393006..3394514	+	1509	WP_003404742.1	carotenoid oxygenase family protein	-
TBXG_RS16045	3394681..3394911	+	231	WP_003898642.1	hypothetical protein	-
TBXG_RS16050	3395046..3395495	-	450	WP_003404738.1	hypothetical protein	-
TBXG_RS16055	3395560..3396333	-	774	WP_003404735.1	VOC family protein	-
TBXG_RS16060	3396431..3396865	-	435	WP_003404726.1	SRPBCC family protein	Toxin
TBXG_RS16065	3396871..3397050	-	180	WP_003898641.1	antitoxin	Antitoxin
TBXG_RS22570	3397176..3397334	-	159	WP_003404720.1	hypothetical protein	-
TBXG_RS16070	3397607..3400000	-	2394	WP_003898639.1	cation-translocating P-type ATPase	-
TBXG_RS16075	3399997..3401577	-	1581	WP_003909313.1	serine hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T27992 WP_003404726.1 NC_018078:c3396865-3396431 [Mycobacterium tuberculosis KZN 605]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T27992 NC_018078:c3396865-3396431 [Mycobacterium tuberculosis KZN 605]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6403.07 Da
• Isoelectric Point: 4.5393

>AT27992 WP_003898641.1 NC_018078:c3397050-3396871 [Mycobacterium tuberculosis KZN 605]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLHDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT27992 NC_018078:c3397050-3396871 [Mycobacterium tuberculosis KZN 605]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCATGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Antitoxin

• AlphaFold DB: A0A045KDK5