Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 2426462..2427038 Replicon chromosome
Accession NZ_CP124372
Organism Escherichia coli strain AVS0151

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag QJP92_RS12410 Protein ID WP_001295743.1
Coordinates 2426751..2427038 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag QJP92_RS12405 Protein ID WP_000063148.1
Coordinates 2426462..2426764 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QJP92_RS12390 (2423058) 2423058..2425208 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
QJP92_RS12395 (2425258) 2425258..2425461 + 204 WP_000467859.1 YbdD/YjiX family protein -
QJP92_RS12400 (2425472) 2425472..2426428 + 957 WP_001295745.1 GTPase -
QJP92_RS12405 (2426462) 2426462..2426764 - 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
QJP92_RS12410 (2426751) 2426751..2427038 - 288 WP_001295743.1 BrnT family toxin Toxin
QJP92_RS12415 (2427235) 2427235..2428401 + 1167 WP_000800831.1 restriction endonuclease -
QJP92_RS12420 (2428465) 2428465..2430084 + 1620 WP_001029745.1 class I SAM-dependent DNA methyltransferase -
QJP92_RS12425 (2430074) 2430074..2431378 + 1305 WP_000535012.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T279893 WP_001295743.1 NZ_CP124372:c2427038-2426751 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT279893 WP_000063148.1 NZ_CP124372:c2426764-2426462 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References