Detailed information of TA system TA027988

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3171964..3172532
• Replicon: chromosome
• Accession: NC_018078
• Organism: Mycobacterium tuberculosis KZN 605

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: TBXG_RS14935
• Protein ID: WP_003405865.1
• Coordinates: 3171964..3172338 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: TBXG_RS14940
• Protein ID: WP_003405863.1
• Coordinates: 3172335..3172532 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBXG_RS14900	3168377..3169084	+	708	Protein_2919	adenylate/guanylate cyclase domain-containing protein	-
TBXG_RS22530	3169036..3169245	-	210	WP_003911400.1	hypothetical protein	-
TBXG_RS14910	3169267..3169977	+	711	WP_003911399.1	hypothetical protein	-
TBXG_RS14915	3169992..3170315	-	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
TBXG_RS14920	3170558..3170833	+	276	WP_003405867.1	hypothetical protein	-
TBXG_RS14925	3170760..3170945	-	186	WP_003901093.1	hypothetical protein	-
TBXG_RS14930	3171063..3171761	-	699	WP_003898733.1	hypothetical protein	-
TBXG_RS14935	3171964..3172338	-	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
TBXG_RS14940	3172335..3172532	-	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
TBXG_RS14945	3172620..3173693	-	1074	WP_003898732.1	redox-regulated ATPase YchF	-
TBXG_RS14950	3173756..3174739	+	984	WP_003898731.1	hypothetical protein	-
TBXG_RS14955	3174756..3175763	-	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
TBXG_RS14960	3175853..3176491	+	639	WP_003898730.1	lipid droplet-associated protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T27988 WP_003405865.1 NC_018078:c3172338-3171964 [Mycobacterium tuberculosis KZN 605]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T27988 NC_018078:c3172338-3171964 [Mycobacterium tuberculosis KZN 605]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT27988 WP_003405863.1 NC_018078:c3172532-3172335 [Mycobacterium tuberculosis KZN 605]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT27988 NC_018078:c3172532-3172335 [Mycobacterium tuberculosis KZN 605]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07