Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 67995..68520 Replicon plasmid pAVS0465-A
Accession NZ_CP124368
Organism Escherichia coli strain AVS0465

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag QJP75_RS25825 Protein ID WP_001159868.1
Coordinates 67995..68300 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag QJP75_RS25830 Protein ID WP_000813634.1
Coordinates 68302..68520 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QJP75_RS25810 (63905) 63905..65071 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
QJP75_RS25815 (65659) 65659..66414 - 756 WP_000852146.1 replication initiation protein RepE -
QJP75_RS25820 (67188) 67188..67994 - 807 WP_000016982.1 site-specific integrase -
QJP75_RS25825 (67995) 67995..68300 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
QJP75_RS25830 (68302) 68302..68520 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
QJP75_RS25835 (69066) 69066..69578 + 513 WP_000151784.1 hypothetical protein -
QJP75_RS25840 (69612) 69612..70733 - 1122 WP_012783980.1 DUF3800 domain-containing protein -
QJP75_RS25845 (70825) 70825..71250 + 426 WP_000422741.1 transposase -
QJP75_RS25850 (71247) 71247..71597 + 351 WP_000624722.1 IS66 family insertion sequence element accessory protein TnpB -
QJP75_RS25855 (71628) 71628..73241 + 1614 WP_000080195.1 IS66-like element ISEc23 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid qacE / sul1 / mph(A) / dfrA17 / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr - 1..103076 103076


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T279879 WP_001159868.1 NZ_CP124368:c68300-67995 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT279879 WP_000813634.1 NZ_CP124368:c68520-68302 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References