Detailed information of TA system TA027987

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3027097..3027785
• Replicon: chromosome
• Accession: NC_018078
• Organism: Mycobacterium tuberculosis KZN 605

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0THR7
• Locus tag: TBXG_RS14270
• Protein ID: WP_003406304.1
• Coordinates: 3027097..3027528 (-)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0THR6
• Locus tag: TBXG_RS14275
• Protein ID: WP_003406302.1
• Coordinates: 3027525..3027785 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBXG_RS14245	3022819..3023088	+	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
TBXG_RS14250	3023085..3023378	+	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	-
TBXG_RS14255	3023435..3024265	+	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
TBXG_RS14260	3024346..3025206	-	861	WP_003900301.1	hypothetical protein	-
TBXG_RS14265	3025386..3027074	+	1689	WP_003910308.1	PE family protein	-
TBXG_RS14270	3027097..3027528	-	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	Toxin
TBXG_RS14275	3027525..3027785	-	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	Antitoxin
TBXG_RS14280	3027861..3028850	-	990	WP_003406301.1	malate dehydrogenase	-
TBXG_RS14285	3029021..3030121	+	1101	WP_003406300.1	magnesium/cobalt transporter CorA	-
TBXG_RS14290	3030198..3031379	-	1182	WP_003406299.1	trehalose ABC transporter ATP-binding protein SugC	-
TBXG_RS14295	3031384..3032208	-	825	WP_003900298.1	trehalose ABC transporter permease SugB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15873.08 Da
• Isoelectric Point: 5.8838

>T27987 WP_003406304.1 NC_018078:c3027528-3027097 [Mycobacterium tuberculosis KZN 605]
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE

Nucleotide

• Length: 432 bp

>T27987 NC_018078:c3027528-3027097 [Mycobacterium tuberculosis KZN 605]
GTGATCATCCCTGACATCAATCTGCTGCTCTACGCGGTCATCACCGGATTCCCGCAGCACCGGCGCGCGCATGCGTGGTG
GCAAGACACCGTCAACGGCCACACCCGTATCGGGCTGACGTATCCGGCGTTGTTCGGGTTCCTACGGATCGCCACCAGTG
CCCGCGTGCTCGCCGCGCCACTGCCAACCGCGGATGCGATCGCCTATGTGCGCGAGTGGCTTTCGCAGCCGAACGTGGAC
CTACTCACGGCGGGTCCGCGCCACCTGGACATCGCGTTGGGCCTGCTCGACAAGCTCGGCACAGCCAGCCACCTAACCAC
CGATGTGCAACTGGCCGCCTACGGCATCGAATACGACGCCGAGATCCATTCCAGTGACACCGACTTTGCCCGATTCGCCG
ATCTGAAGTGGACCGACCCGTTGCGCGAATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9918.25 Da
• Isoelectric Point: 6.4755

>AT27987 WP_003406302.1 NC_018078:c3027785-3027525 [Mycobacterium tuberculosis KZN 605]
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAGFNKLADELEDEALLD
ATRRAR

Nucleotide

• Length: 261 bp

>AT27987 NC_018078:c3027785-3027525 [Mycobacterium tuberculosis KZN 605]
ATGCGCACCACCTTGACGCTCGATGACGACGTCGTCCGGCTGGTCGAAGACGCAGTGCATCGCGAACGCCGCCCGATGAA
GCAGGTCATCAACGATGCGCTGCGCAGAGCGCTGGCGCCGCCGGTGAAACGGCAGGAGCAGTATCGGTTGGAGCCGCATG
AGTCGGCTGTGCGTTCCGGGTTGGATCTGGCCGGCTTCAACAAGTTGGCCGACGAACTGGAGGATGAGGCGCTGCTGGAT
GCCACGCGTCGGGCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0THR7

Antitoxin

• AlphaFold DB: G0THR6