Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 2647965..2648578 Replicon chromosome
Accession NC_018078
Organism Mycobacterium tuberculosis KZN 605

Toxin (Protein)


Gene name vapC Uniprot ID P64880
Locus tag TBXG_RS12580 Protein ID WP_003407786.1
Coordinates 2647965..2648369 (-) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0E8UJ46
Locus tag TBXG_RS12585 Protein ID WP_009935474.1
Coordinates 2648375..2648578 (-) Length 68 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TBXG_RS12570 2643916..2646213 + 2298 WP_003407790.1 malto-oligosyltrehalose synthase -
TBXG_RS12575 2646206..2647948 + 1743 WP_003407788.1 malto-oligosyltrehalose trehalohydrolase -
TBXG_RS12580 2647965..2648369 - 405 WP_003407786.1 type II toxin-antitoxin system toxin ribonuclease VapC11 Toxin
TBXG_RS12585 2648375..2648578 - 204 WP_009935474.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
TBXG_RS12590 2648631..2649920 - 1290 WP_003407781.1 threonine ammonia-lyase -
TBXG_RS12595 2649955..2650401 - 447 WP_003407780.1 F420H(2)-dependent quinone reductase -
TBXG_RS12600 2650411..2651559 - 1149 Protein_2470 MMPL family transporter -
TBXG_RS12605 2651743..2652351 - 609 WP_003407773.1 TetR/AcrR family transcriptional regulator -
TBXG_RS12610 2652419..2652796 - 378 WP_003407771.1 fumarate reductase subunit FrdD -
TBXG_RS12615 2652793..2653173 - 381 WP_003407768.1 fumarate reductase subunit C -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-124)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(2-44)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 14650.76 Da        Isoelectric Point: 5.6587

>T27982 WP_003407786.1 NC_018078:c2648369-2647965 [Mycobacterium tuberculosis KZN 605]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Download         Length: 405 bp

>T27982 NC_018078:c2648369-2647965 [Mycobacterium tuberculosis KZN 605]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin


Download         Length: 68 a.a.        Molecular weight: 7501.48 Da        Isoelectric Point: 4.3951

>AT27982 WP_009935474.1 NC_018078:c2648578-2648375 [Mycobacterium tuberculosis KZN 605]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Download         Length: 204 bp

>AT27982 NC_018078:c2648578-2648375 [Mycobacterium tuberculosis KZN 605]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6A7V

Antitoxin

Source ID Structure
AlphaFold DB A0A0E8UJ46

References