Detailed information of TA system TA027979

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2227607..2228310
• Replicon: chromosome
• Accession: NC_018078
• Organism: Mycobacterium tuberculosis KZN 605

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: TBXG_RS10725
• Protein ID: WP_003409778.1
• Coordinates: 2227981..2228310 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: TBXG_RS21290
• Protein ID: WP_003409780.1
• Coordinates: 2227607..2227984 (+)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBXG_RS10680	2222765..2222956	+	192	WP_003409876.1	hypothetical protein	-
TBXG_RS10685	2222977..2223879	+	903	WP_003899097.1	hypothetical protein	-
TBXG_RS10690	2223890..2224240	+	351	WP_003899096.1	hypothetical protein	-
TBXG_RS10695	2224354..2224536	+	183	WP_003409870.1	hypothetical protein	-
TBXG_RS10700	2224529..2224930	-	402	WP_003409869.1	hypothetical protein	-
TBXG_RS10705	2224994..2225446	+	453	WP_003899095.1	lipoprotein	-
TBXG_RS10710	2225601..2226965	-	1365	WP_003903691.1	HNH endonuclease	-
TBXG_RS10715	2227020..2227610	+	591	WP_003409784.1	SEC-C domain-containing protein	-
TBXG_RS21290	2227607..2227984	+	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
TBXG_RS10725	2227981..2228310	+	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
TBXG_RS10730	2228520..2229290	-	771	WP_003409775.1	SDR family oxidoreductase	-
TBXG_RS10735	2229287..2230348	-	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
TBXG_RS10740	2230345..2230860	-	516	WP_003409718.1	flavin reductase family protein	-
TBXG_RS10745	2230857..2231927	-	1071	WP_003899091.1	epoxide hydrolase EphB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T27979 WP_003409778.1 NC_018078:2227981-2228310 [Mycobacterium tuberculosis KZN 605]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T27979 NC_018078:2227981-2228310 [Mycobacterium tuberculosis KZN 605]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT27979 WP_003409780.1 NC_018078:2227607-2227984 [Mycobacterium tuberculosis KZN 605]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT27979 NC_018078:2227607-2227984 [Mycobacterium tuberculosis KZN 605]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8